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10.1039/d1cb00169h

http://scihub22266oqcxt.onion/10.1039/d1cb00169h
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34977572!8637828!34977572
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suck abstract from ncbi


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pmid34977572      RSC+Chem+Biol 2021 ; 2 (6): 1580-1589
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  • An overview of methods for the structural and functional mapping of epitopes recognized by anti-SARS-CoV-2 antibodies #MMPMID34977572
  • Francino-Urdaniz IM; Whitehead TA
  • RSC Chem Biol 2021[Dec]; 2 (6): 1580-1589 PMID34977572show ga
  • This mini-review presents a critical survey of techniques used for epitope mapping on the SARS-CoV-2 Spike protein. The sequence and structures for common neutralizing and non-neutralizing epitopes on the Spike protein are described as determined by X-ray crystallography, electron microscopy and linear peptide epitope mapping, among other methods. An additional focus of this mini-review is an analytical appraisal of different deep mutational scanning workflows for conformational epitope mapping and identification of mutants on the Spike protein which escape antibody neutralization. Such a focus is necessary as a critical review of deep mutational scanning for conformational epitope mapping has not been published. A perspective is presented on the use of different epitope determination methods for development of broadly potent antibody therapies and vaccines against SARS-CoV-2.
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