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10.3390/v13122553

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34960822!8705234!34960822
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suck abstract from ncbi


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pmid34960822      Viruses 2021 ; 13 (12): ä
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  • Microarray-Based Detection of Antibodies against SARS-CoV-2 Proteins, Common Respiratory Viruses and Type I Interferons #MMPMID34960822
  • Savvateeva E; Filippova M; Valuev-Elliston V; Nuralieva N; Yukina M; Troshina E; Baklaushev V; Ivanov A; Gryadunov D
  • Viruses 2021[Dec]; 13 (12): ä PMID34960822show ga
  • A microarray-based assay to detect IgG and IgM antibodies against betacoronaviruses (SARS-CoV-2, SARS, MERS, OC43, and HKU1), other respiratory viruses and type I interferons (IFN-Is) was developed. This multiplex assay was applied to track antibody cross-reactivity due to previous contact with similar viruses and to identify antibodies against IFN-Is as the markers for severe COVID-19. In total, 278 serum samples from convalescent plasma donors, COVID-19 patients in the intensive care unit (ICU) and patients who recovered from mild/moderate COVID-19, vaccine recipients, prepandemic and pandemic patients with autoimmune endocrine disorders, and a heterogeneous prepandemic cohort including healthy individuals and chronically ill patients were analyzed. The anti-SARS-CoV-2 microarray results agreed well with the ELISA results. Regarding ICU patients, autoantibodies against IFN-Is were detected in 10.5% of samples, and 10.5% of samples were found to simultaneously contain IgM antibodies against more than two different viruses. Cross-reactivity between IgG against the SARS-CoV-2 nucleocapsid and IgG against the OC43 and HKU1 spike proteins was observed, resulting in positive signals for the SARS-CoV-2 nucleocapsid in prepandemic samples from patients with autoimmune endocrine disorders. The presence of IgG against the SARS-CoV-2 nucleocapsid in the absence of IgG against the SARS-CoV-2 spike RBD should be interpreted with caution.
  • |Antibodies, Viral/blood/*immunology[MESH]
  • |Antigens, Viral/immunology[MESH]
  • |Autoantibodies/blood/immunology[MESH]
  • |COVID-19 Serological Testing[MESH]
  • |COVID-19/immunology[MESH]
  • |Cross Reactions[MESH]
  • |Humans[MESH]
  • |Immunoglobulin G/blood/immunology[MESH]
  • |Immunoglobulin M/blood/immunology[MESH]
  • |Interferon Type I/*immunology[MESH]
  • |Protein Array Analysis[MESH]
  • |Respiratory Tract Diseases/immunology/virology[MESH]
  • |SARS-CoV-2/*immunology[MESH]


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