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10.1016/j.bbadis.2021.166322 http://scihub22266oqcxt.onion/10.1016/j.bbadis.2021.166322 34920080!8669938!34920080     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biochim+Biophys+Acta+Mol+Basis+Dis 2022 ; 1868 (3): 166322 Nephropedia Template TP {{tp|p=34920080|t=2022. The spike protein of SARS-CoV-2 induces heme oxygenase-1: Pathophysiologic implications |pdf=|usr=}}{{34920080}} gab.com Text The spike protein of SARS-CoV-2 induces heme oxygenase-1: Pathophysiologic implications JO: Biochim Biophys Acta Mol Basis Dis http://www.kidney.de/pget.php?&tw=1&pmid=34920080 #FOAvip BACKGROUND: Acute kidney injury (AKI) is both a consequence and determinant of outcomes in COVID-19. The kidney is one of the major organs infected by the causative virus, SARS-CoV-2. Viral entry into cells requires the viral spike protein, and both the virus and its spike protein appear in the urine of COVID-19 patients with AKI. We examined the effects of transfecting the viral spike protein of SARS-CoV-2 in kidney cell lines. METHODS: HEK293, HEK293-ACE2(+) (stably overexpressing ACE2), and Vero E6 cells having endogenous ACE2 were transfected with SARS-CoV-2 spike or control plasmid. Assessment of gene and protein expression, and syncytia formation was performed, and the effects of quercetin on syncytia formation examined. FINDINGS: Spike transfection in HEK293-ACE2(+) cells caused syncytia formation, cellular sloughing, and focal denudation of the cell monolayer; transfection in Vero E6 cells also caused syncytia formation. Spike expression upregulated potentially nephrotoxic genes (TNF-alpha, MCP-1, and ICAM1). Spike upregulated the cytoprotective gene HO-1 and relevant signaling pathways (p-Akt, p-STAT3, and p-p38). Quercetin, an HO-1 inducer, reduced syncytia formation and spike protein expression. INTERPRETATION: The major conclusions of the study are: 1) Spike protein expression in kidney cells provides a relevant model for the study of maladaptive and adaptive responses germane to AKI in COVID-19; 2) such spike protein expression upregulates HO-1; and 3) quercetin, an HO-1 inducer, may provide a clinically relevant/feasible protective strategy in AKI occurring in the setting of COVID-19. FUNDING: R01-DK119167 (KAN), R01-AI100911 (JPG), P30-DK079337; R01-DK059600 (AA). Twit Text FOAVip The spike protein of SARS-CoV-2 induces heme oxygenase-1: Pathophysiologic implications ????Biochim Biophys Acta Mol Basis Dis http://www.kidney.de/pget.php?&tw=1&pmid=34920080 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34920080 #FOAvip English Wikipedia <ref>{{Cite pmid|34920080}}</ref> The spike protein of SARS-CoV-2 induces heme oxygenase-1: Pathophysiologic implications #MMPMID34920080 Singh RD; Barry MA; Croatt AJ; Ackerman AW; Grande JP; Diaz RM; Vile RG; Agarwal A; Nath KA Biochim Biophys Acta Mol Basis Dis 2022[Mar]; 1868 (3): 166322 PMID34920080show ga BACKGROUND: Acute kidney injury (AKI) is both a consequence and determinant of outcomes in COVID-19. The kidney is one of the major organs infected by the causative virus, SARS-CoV-2. Viral entry into cells requires the viral spike protein, and both the virus and its spike protein appear in the urine of COVID-19 patients with AKI. We examined the effects of transfecting the viral spike protein of SARS-CoV-2 in kidney cell lines. METHODS: HEK293, HEK293-ACE2(+) (stably overexpressing ACE2), and Vero E6 cells having endogenous ACE2 were transfected with SARS-CoV-2 spike or control plasmid. Assessment of gene and protein expression, and syncytia formation was performed, and the effects of quercetin on syncytia formation examined. FINDINGS: Spike transfection in HEK293-ACE2(+) cells caused syncytia formation, cellular sloughing, and focal denudation of the cell monolayer; transfection in Vero E6 cells also caused syncytia formation. Spike expression upregulated potentially nephrotoxic genes (TNF-alpha, MCP-1, and ICAM1). Spike upregulated the cytoprotective gene HO-1 and relevant signaling pathways (p-Akt, p-STAT3, and p-p38). Quercetin, an HO-1 inducer, reduced syncytia formation and spike protein expression. INTERPRETATION: The major conclusions of the study are: 1) Spike protein expression in kidney cells provides a relevant model for the study of maladaptive and adaptive responses germane to AKI in COVID-19; 2) such spike protein expression upregulates HO-1; and 3) quercetin, an HO-1 inducer, may provide a clinically relevant/feasible protective strategy in AKI occurring in the setting of COVID-19. FUNDING: R01-DK119167 (KAN), R01-AI100911 (JPG), P30-DK079337; R01-DK059600 (AA). |Animals[MESH] |COVID-19/*metabolism/virology[MESH] |Cell Line[MESH] |Chlorocebus aethiops[MESH] |HEK293 Cells[MESH] |Heme Oxygenase-1/*metabolism[MESH] |Host-Pathogen Interactions/drug effects/physiology[MESH] |Humans[MESH] |Protein Binding/drug effects/physiology[MESH] |Quercetin/pharmacology[MESH] |SARS-CoV-2/*pathogenicity[MESH] |Signal Transduction/drug effects/physiology[MESH] |Spike Glycoprotein, Coronavirus/*metabolism[MESH] |Up-Regulation/drug effects/physiology[MESH] |Vero Cells[MESH]The spike protein of SARS-CoV-2 induces heme oxygenase-1: Pathophysiol(epmc).pdf DeepDyve Pubget Overpricing