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10.1038/s41598-021-03273-0 http://scihub22266oqcxt.onion/10.1038/s41598-021-03273-0 34887501!8660863!34887501     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34887501&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 23713 Nephropedia Template TP {{tp|p=34887501|t=2021. The potential of COVID-19 patients sera to cause antibody-dependent enhancement of infection and IL-6 production |pdf=|usr=}}{{34887501}} gab.com Text The potential of COVID-19 patients sera to cause antibody-dependent enhancement of infection and IL-6 production JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34887501 #FOAvip Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many vaccine trials have been initiated. An important goal of vaccination is the development of neutralizing antibody (Ab) against SARS-CoV-2. However, the possible induction of antibody-dependent enhancement (ADE) of infection, which is known for other coronaviruses and dengue virus infections, is a particular concern in vaccine development. Here, we demonstrated that human iPS cell-derived, immortalized, and ACE2- and TMPRSS2-expressing myeloid cell lines are useful as host cells for SARS-CoV-2 infection. The established cell lines were cloned and screened based on their function in terms of susceptibility to SARS-CoV-2-infection or IL-6 productivity. Using the resulting K-ML2 (AT) clone 35 for SARS-CoV-2-infection or its subclone 35-40 for IL-6 productivity, it was possible to evaluate the potential of sera from severe COVID-19 patients to cause ADE and to stimulate IL-6 production upon infection with SARS-CoV-2. Twit Text FOAVip The potential of COVID-19 patients sera to cause antibody-dependent enhancement of infection and IL-6 production ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34887501 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34887501 #FOAvip English Wikipedia <ref>{{Cite pmid|34887501}}</ref> The potential of COVID-19 patients sera to cause antibody-dependent enhancement of infection and IL-6 production #MMPMID34887501 Shimizu J; Sasaki T; Yamanaka A; Ichihara Y; Koketsu R; Samune Y; Cruz P; Sato K; Tanga N; Yoshimura Y; Murakami A; Yamada M; Itoi K; Nakayama EE; Miyazaki K; Shioda T Sci Rep 2021[Dec]; 11 (1): 23713 PMID34887501show ga Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), many vaccine trials have been initiated. An important goal of vaccination is the development of neutralizing antibody (Ab) against SARS-CoV-2. However, the possible induction of antibody-dependent enhancement (ADE) of infection, which is known for other coronaviruses and dengue virus infections, is a particular concern in vaccine development. Here, we demonstrated that human iPS cell-derived, immortalized, and ACE2- and TMPRSS2-expressing myeloid cell lines are useful as host cells for SARS-CoV-2 infection. The established cell lines were cloned and screened based on their function in terms of susceptibility to SARS-CoV-2-infection or IL-6 productivity. Using the resulting K-ML2 (AT) clone 35 for SARS-CoV-2-infection or its subclone 35-40 for IL-6 productivity, it was possible to evaluate the potential of sera from severe COVID-19 patients to cause ADE and to stimulate IL-6 production upon infection with SARS-CoV-2. |*Antibody-Dependent Enhancement[MESH] |Angiotensin-Converting Enzyme 2/metabolism[MESH] |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/immunology[MESH] |COVID-19/*immunology/*metabolism[MESH] |Cell Line[MESH] |Humans[MESH] |Interleukin-6/*metabolism[MESH] |Myeloid Cells/immunology/metabolism[MESH] |Patients[MESH] |SARS-CoV-2/*immunology[MESH]The potential of COVID-19 patients sera to cause antibody-dependent e(epmc).pdf DeepDyve Pubget Overpricing