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10.1016/j.xpro.2021.101051

http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.101051
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34877545!8639449!34877545
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suck abstract from ncbi


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pmid34877545      STAR+Protoc 2022 ; 3 (1): 101051
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  • Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS) #MMPMID34877545
  • Sharma N; Bhat SH; Tripathi G; Yadav M; Mathew B; Bindal V; Sharma S; Gupta E; Maras JS; Sarin SK
  • STAR Protoc 2022[Mar]; 3 (1): 101051 PMID34877545show ga
  • Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
  • |COVID-19/*diagnosis/metabolism[MESH]
  • |Chromatography, High Pressure Liquid/*methods[MESH]
  • |Computational Biology[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Gene Expression Profiling[MESH]
  • |Genetic Techniques[MESH]
  • |Humans[MESH]
  • |Influenza A Virus, H1N1 Subtype/metabolism/pathogenicity[MESH]
  • |Mass Spectrometry/methods[MESH]
  • |Metabolome[MESH]
  • |Metabolomics/*methods[MESH]
  • |Reference Standards[MESH]
  • |SARS-CoV-2/metabolism/pathogenicity[MESH]


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