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Global metabolome profiling of COVID-19 respiratory specimen using high-resolution mass spectrometry (HRMS) #MMPMID34877545
Sharma N; Bhat SH; Tripathi G; Yadav M; Mathew B; Bindal V; Sharma S; Gupta E; Maras JS; Sarin SK
STAR Protoc 2022[Mar]; 3 (1): 101051 PMID34877545show ga
Here we describe a protocol for identifying metabolites in respiratory specimens of patients that are SARS-CoV-2 positive, SARS-CoV-2 negative, or H1N1 positive. This protocol provides step-by-step instructions on sample collection from patients, followed by metabolite extraction. We use ultra-high-pressure liquid chromatography (UHPLC) coupled with high-resolution mass spectrometry (HRMS) for data acquisition and describe the steps for data analysis. The protocol was standardized with specific customization for SARS-CoV-2-containing respiratory specimens. For complete details on the use and execution of this protocol, please refer to Maras et al. (2021).
|COVID-19/*diagnosis/metabolism[MESH]
|Chromatography, High Pressure Liquid/*methods[MESH]
|Computational Biology[MESH]
|Diagnostic Tests, Routine[MESH]
|Gene Expression Profiling[MESH]
|Genetic Techniques[MESH]
|Humans[MESH]
|Influenza A Virus, H1N1 Subtype/metabolism/pathogenicity[MESH]