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10.1038/s41598-021-02938-0 http://scihub22266oqcxt.onion/10.1038/s41598-021-02938-0 34876637!8651634!34876637     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Sci+Rep 2021 ; 11 (1): 23558 Nephropedia Template TP {{tp|p=34876637|t=2021. Determination of murine norovirus aerosol concentration during toilet flushing |pdf=|usr=}}{{34876637}} gab.com Text Determination of murine norovirus aerosol concentration during toilet flushing JO: Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34876637 #FOAvip Murine norovirus (MNV) was used as a surrogate for human viral pathogens (e.g., norovirus) to determine if toilet flushing resulted in the aerosolization of virus. A flushometer type toilet was seeded with a viral solution of 10(5) and 10(6) PFU mL(-1) of MNV and then flushed. Upon flushing, two bioaerosol samplers were activated to collect aerosolized MNV. Prior to the experiment, two optical particle counters monitored particle size and number distribution of aerosol produced from flushing a toilet across height, position, and side. The location with the highest mean particle concentration, was behind the toilet and 0.15 m above the toilet bowl rim, which is where bioaerosol sampling occurred. Bioaerosol and toilet water samples were collected, extracted and then quantified using RT-ddPCR. The concentration of MNV collected after seeding the toilet water ranged from 2.18 x 10(5) to 9.65 x 10(6) total copies of MNV. Positive samples of airborne MNV were detected with collected concentrations ranging from 383 to 684 RNA copies/m(3) of air. This study provides evidence that viral pathogens may be aerosolized when a toilet is flushed. Furthermore, the MNV used in this study is a model organism for human norovirus and may be generalizable to other viral pathogens (e.g., coronavirus). This study suggests that virus is aerosolized from toilet flushing and may contribute to human exposure to viral pathogens. Twit Text FOAVip Determination of murine norovirus aerosol concentration during toilet flushing ????Sci Rep http://www.kidney.de/pget.php?&tw=1&pmid=34876637 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34876637 #FOAvip English Wikipedia <ref>{{Cite pmid|34876637}}</ref> Determination of murine norovirus aerosol concentration during toilet flushing #MMPMID34876637 Boles C; Brown G; Nonnenmann M Sci Rep 2021[Dec]; 11 (1): 23558 PMID34876637show ga Murine norovirus (MNV) was used as a surrogate for human viral pathogens (e.g., norovirus) to determine if toilet flushing resulted in the aerosolization of virus. A flushometer type toilet was seeded with a viral solution of 10(5) and 10(6) PFU mL(-1) of MNV and then flushed. Upon flushing, two bioaerosol samplers were activated to collect aerosolized MNV. Prior to the experiment, two optical particle counters monitored particle size and number distribution of aerosol produced from flushing a toilet across height, position, and side. The location with the highest mean particle concentration, was behind the toilet and 0.15 m above the toilet bowl rim, which is where bioaerosol sampling occurred. Bioaerosol and toilet water samples were collected, extracted and then quantified using RT-ddPCR. The concentration of MNV collected after seeding the toilet water ranged from 2.18 x 10(5) to 9.65 x 10(6) total copies of MNV. Positive samples of airborne MNV were detected with collected concentrations ranging from 383 to 684 RNA copies/m(3) of air. This study provides evidence that viral pathogens may be aerosolized when a toilet is flushed. Furthermore, the MNV used in this study is a model organism for human norovirus and may be generalizable to other viral pathogens (e.g., coronavirus). This study suggests that virus is aerosolized from toilet flushing and may contribute to human exposure to viral pathogens. |Aerosols/*analysis[MESH] |Air Microbiology[MESH] |Animals[MESH] |Bathroom Equipment/*virology[MESH] |Mice[MESH] |Norovirus/genetics/*isolation & purification[MESH] |RNA, Viral/metabolism[MESH]Determination of murine norovirus aerosol concentration during toilet (epmc).pdf DeepDyve Pubget Overpricing