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10.1016/j.xpro.2021.101045

http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.101045
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34870243!8626227!34870243
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suck abstract from ncbi


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pmid34870243      STAR+Protoc 2022 ; 3 (1): 101045
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  • Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis #MMPMID34870243
  • Tripathi G; Sharma N; Bindal V; Yadav M; Mathew B; Sharma S; Gupta E; Singh Maras J; Sarin SK
  • STAR Protoc 2022[Mar]; 3 (1): 101045 PMID34870243show ga
  • In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).
  • |COVID-19/*diagnosis/metabolism[MESH]
  • |Chromatography, Liquid/methods[MESH]
  • |Computational Biology[MESH]
  • |Diagnostic Tests, Routine[MESH]
  • |Gene Expression Profiling[MESH]
  • |Genetic Techniques[MESH]
  • |Genome, Viral/genetics[MESH]
  • |Genomics/*methods[MESH]
  • |Humans[MESH]
  • |Influenza A Virus, H1N1 Subtype/metabolism/pathogenicity[MESH]
  • |Peptides[MESH]
  • |Proteome[MESH]
  • |Proteomics/*methods[MESH]
  • |SARS-CoV-2/metabolism/pathogenicity[MESH]
  • |Specimen Handling/methods[MESH]
  • |Tandem Mass Spectrometry/methods[MESH]


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