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Protocol for global proteome, virome, and metaproteome profiling of respiratory specimen (VTM) in COVID-19 patient by LC-MS/MS-based analysis #MMPMID34870243
Tripathi G; Sharma N; Bindal V; Yadav M; Mathew B; Sharma S; Gupta E; Singh Maras J; Sarin SK
STAR Protoc 2022[Mar]; 3 (1): 101045 PMID34870243show ga
In this protocol, we describe global proteome profiling for the respiratory specimen of COVID-19 patients, patients suspected with COVID-19, and H1N1 patients. In this protocol, details for identifying host, viral, or bacterial proteome (Meta-proteome) are provided. Major steps of the protocol include virus inactivation, protein quantification and digestion, desalting of peptides, high-resolution mass spectrometry (HRMS)-based analysis, and downstream bioinformatics analysis. For complete details on the use and execution of this profile, please refer to Maras et al. (2021).
|COVID-19/*diagnosis/metabolism[MESH]
|Chromatography, Liquid/methods[MESH]
|Computational Biology[MESH]
|Diagnostic Tests, Routine[MESH]
|Gene Expression Profiling[MESH]
|Genetic Techniques[MESH]
|Genome, Viral/genetics[MESH]
|Genomics/*methods[MESH]
|Humans[MESH]
|Influenza A Virus, H1N1 Subtype/metabolism/pathogenicity[MESH]
free
free
free
STAR+Protoc 2022 ; 3 (1): 101045
