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10.1186/s13059-021-02526-5

http://scihub22266oqcxt.onion/10.1186/s13059-021-02526-5
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suck abstract from ncbi


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pmid34844647      Genome+Biol 2021 ; 22 (1): 324
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  • treekoR: identifying cellular-to-phenotype associations by elucidating hierarchical relationships in high-dimensional cytometry data #MMPMID34844647
  • Chan A; Jiang W; Blyth E; Yang J; Patrick E
  • Genome Biol 2021[Nov]; 22 (1): 324 PMID34844647show ga
  • High-throughput single-cell technologies hold the promise of discovering novel cellular relationships with disease. However, analytical workflows constructed for these technologies to associate cell proportions with disease often employ unsupervised clustering techniques that overlook the valuable hierarchical structures that have been used to define cell types. We present treekoR, a framework that empirically recapitulates these structures, facilitating multiple quantifications and comparisons of cell type proportions. Our results from twelve case studies reinforce the importance of quantifying proportions relative to parent populations in the analyses of cytometry data - as failing to do so can lead to missing important biological insights.
  • |*Phenotype[MESH]
  • |CD8 Antigens[MESH]
  • |CD8-Positive T-Lymphocytes[MESH]
  • |COVID-19[MESH]
  • |Cluster Analysis[MESH]
  • |Flow Cytometry/*methods[MESH]
  • |Gene Expression Profiling[MESH]
  • |High-Throughput Nucleotide Sequencing[MESH]
  • |Humans[MESH]


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