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10.3390/cells10113159 http://scihub22266oqcxt.onion/10.3390/cells10113159 34831382!8622730!34831382     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cells 2021 ; 10 (11): ä Nephropedia Template TP {{tp|p=34831382|t=2021. Identification of Cellular Factors Required for SARS-CoV-2 Replication |pdf=|usr=}}{{34831382}} gab.com Text Identification of Cellular Factors Required for SARS-CoV-2 Replication JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=34831382 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for the COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild respiratory tract infection to severe disease with lung injury, multiorgan failure, and death. SARS-CoV-2 is the third animal coronavirus to emerge in humans in the 21st century, and coronaviruses appear to possess a unique ability to cross borders between species and infect a wide range of organisms. This is somewhat surprising as, except for the requirement of host cell receptors, cell-pathogen interactions are usually species-specific. Insights into these host-virus interactions will provide a deeper understanding of the process of SARS-CoV-2 infection and provide a means for the design and development of antiviral agents. In this study, we describe a complex analysis of SARS-CoV-2 infection using a genome-wide CRISPR-Cas9 knock-out system in HeLa cells overexpressing entry receptor angiotensin-converting enzyme 2 (ACE2). This platform allows for the identification of factors required for viral replication. This study was designed to include a high number of replicates (48 replicates; 16 biological repeats with 3 technical replicates each) to prevent data instability, remove sources of bias, and allow multifactorial bioinformatic analyses in order to study the resulting interaction network. The results obtained provide an interesting insight into the replication mechanisms of SARS-CoV-2. Twit Text FOAVip Identification of Cellular Factors Required for SARS-CoV-2 Replication ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=34831382 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34831382 #FOAvip English Wikipedia <ref>{{Cite pmid|34831382}}</ref> Identification of Cellular Factors Required for SARS-CoV-2 Replication #MMPMID34831382 Synowiec A; Jedrysik M; Branicki W; Klajmon A; Lei J; Owczarek K; Suo C; Szczepanski A; Wang J; Zhang P; Labaj PP; Pyrc K Cells 2021[Nov]; 10 (11): ä PMID34831382show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the recently emerged virus responsible for the COVID-19 pandemic. Clinical presentation can range from asymptomatic disease and mild respiratory tract infection to severe disease with lung injury, multiorgan failure, and death. SARS-CoV-2 is the third animal coronavirus to emerge in humans in the 21st century, and coronaviruses appear to possess a unique ability to cross borders between species and infect a wide range of organisms. This is somewhat surprising as, except for the requirement of host cell receptors, cell-pathogen interactions are usually species-specific. Insights into these host-virus interactions will provide a deeper understanding of the process of SARS-CoV-2 infection and provide a means for the design and development of antiviral agents. In this study, we describe a complex analysis of SARS-CoV-2 infection using a genome-wide CRISPR-Cas9 knock-out system in HeLa cells overexpressing entry receptor angiotensin-converting enzyme 2 (ACE2). This platform allows for the identification of factors required for viral replication. This study was designed to include a high number of replicates (48 replicates; 16 biological repeats with 3 technical replicates each) to prevent data instability, remove sources of bias, and allow multifactorial bioinformatic analyses in order to study the resulting interaction network. The results obtained provide an interesting insight into the replication mechanisms of SARS-CoV-2. |*Virus Replication[MESH] |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |CRISPR-Cas Systems[MESH] |Computational Biology[MESH] |Genome, Human/genetics[MESH] |HeLa Cells[MESH] |Host-Pathogen Interactions[MESH] |Humans[MESH]Identification of Cellular Factors Required for SARS-CoV-2 Replication(epmc).pdf DeepDyve Pubget Overpricing