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10.3390/cells10113019 http://scihub22266oqcxt.onion/10.3390/cells10113019 34831243!8616302!34831243     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34831243&cmd=llinks): Failed to open stream: HTTP request failed! 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Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes |pdf=|usr=}}{{34831243}} gab.com Text Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=34831243 #FOAvip The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes. Twit Text FOAVip Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=34831243 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34831243 #FOAvip English Wikipedia <ref>{{Cite pmid|34831243}}</ref> Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Primary Human Hepatocytes #MMPMID34831243 Nicolay W; Moeller R; Kahl S; Vondran FWR; Pietschmann T; Kunz S; Gerold G Cells 2021[Nov]; 10 (11): ä PMID34831243show ga The liver is targeted by several human pathogenic RNA viruses for viral replication and dissemination; despite this, the extent of innate immune sensing of RNA viruses by human hepatocytes is insufficiently understood to date. In particular, for highly human tropic viruses such as hepatitis C virus, cell culture models are needed to study immune sensing. However, several human hepatoma cell lines have impaired RNA sensing pathways and fail to mimic innate immune responses in the human liver. Here we compare the RNA sensing properties of six human hepatoma cell lines, namely Huh-6, Huh-7, HepG2, HepG2-HFL, Hep3B, and HepaRG, with primary human hepatocytes. We show that primary liver cells sense RNA through retinoic acid-inducible gene I (RIG-I) like receptor (RLR) and Toll-like receptor 3 (TLR3) pathways. Of the tested cell lines, Hep3B cells most closely mimicked the RLR and TLR3 mediated sensing in primary hepatocytes. This was shown by the expression of RLRs and TLR3 as well as the expression and release of bioactive interferon in primary hepatocytes and Hep3B cells. Our work shows that Hep3B cells partially mimic RNA sensing in primary hepatocytes and thus can serve as in vitro model to study innate immunity to RNA viruses in hepatocytes. |*Immunity, Innate[MESH] |Carcinoma, Hepatocellular/pathology[MESH] |Cell Line, Tumor[MESH] |Cells, Cultured[MESH] |DEAD Box Protein 58/immunology[MESH] |Hepatocytes/*immunology/virology[MESH] |Humans[MESH] |Interferons/immunology[MESH] |Liver Neoplasms/pathology[MESH] |Liver/cytology/immunology/virology[MESH] |RNA Viruses/physiology[MESH] |RNA/*immunology[MESH] |Receptors, Immunologic/immunology[MESH] |Signal Transduction/immunology[MESH] |Toll-Like Receptor 3/immunology[MESH]Characterization of RNA Sensing Pathways in Hepatoma Cell Lines and Pr(epmc).pdf DeepDyve Pubget Overpricing