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Deprecated: Implicit conversion from float 219.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 mBio 2021 ; 12 (6): e0298421 Nephropedia Template TP
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A Previously Undiscovered Circular RNA, circTNFAIP3, and Its Role in Coronavirus Replication #MMPMID34781747
Du L; Wang X; Liu J; Li J; Wang S; Lei J; Zhou J; Gu J
mBio 2021[Dec]; 12 (6): e0298421 PMID34781747show ga
Circular RNAs (circRNAs) are a newly discovered class of noncoding RNAs (ncRNAs) present in various tissues and cells. However, the functions of most circRNAs have not been verified experimentally. Here, using deltacoronavirus as a model, differentially expressed circRNAs in cells with or without deltacoronavirus infection were analyzed by RNA sequencing to characterize the cellular responses to RNA virus infection. More than 57,000 circRNA candidates were detected, and seven significantly dysregulated circRNAs were quantitated by real-time reverse transcription-PCR. We discovered a previously unidentified circRNA derived from the TNFAIP3 gene, named circTNFAIP3, which is distributed and expressed widely in various tissues. RNA viruses, including deltacoronaviruses, rather than DNA viruses tend to activate the expression of endogenous circTNFAIP3. Overexpression of circTNFAIP3 promoted deltacoronavirus replication by reducing the apoptosis, while silencing of circTNFAIP3 inhibited deltacoronavirus replication by enhancing the apoptosis. In summary, our work provides useful circRNA-related information to facilitate investigation of the underlying mechanism of deltacoronavirus infection and identifies a novel circTNFAIP3 that promotes deltacoronavirus replication via regulating apoptosis. IMPORTANCE CircRNAs, a new class of ncRNAs, play important roles in cell growth, neural development, carcinogenesis, and anticarcinogenesis. Porcine deltacoronavirus is an emerging enteropathogenic coronavirus that causes diarrhea, but the role of host circRNAs in regulating its infection is unknown. Here, we performed expression profiling of circRNAs in mock- and deltacoronavirus- infected cells and identified the novel differentially expressed circular RNA circTNFAIP3. We demonstrate that circTNFAIP3 promotes deltacoronavirus replication by inhibiting apoptosis. Our findings first illustrate that circRNA can act as an apoptosis negative regulator during RNA virus infection and help to explore the underlying mechanism of deltacoronavirus infection.
|Apoptosis[MESH]
|Cell Line[MESH]
|Deltacoronavirus/*genetics/physiology[MESH]
|Gene Expression Profiling[MESH]
|HEK293 Cells[MESH]
|High-Throughput Nucleotide Sequencing[MESH]
|Host Microbial Interactions/*genetics[MESH]
|Humans[MESH]
|RNA, Circular/*genetics/immunology[MESH]
|Sequence Analysis, RNA[MESH]
|Tumor Necrosis Factor alpha-Induced Protein 3/*genetics/immunology[MESH]
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mBio 2021 ; 12 (6): e0298421
