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10.1371/journal.pone.0259165 http://scihub22266oqcxt.onion/10.1371/journal.pone.0259165 34762662!8584957!34762662     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+One 2021 ; 16 (11): e0259165 Nephropedia Template TP {{tp|p=34762662|t=2021. Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens |pdf=|usr=}}{{34762662}} gab.com Text Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34762662 #FOAvip The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics. Twit Text FOAVip Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34762662 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34762662 #FOAvip English Wikipedia <ref>{{Cite pmid|34762662}}</ref> Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinical specimens #MMPMID34762662 Bezstarosti K; Lamers MM; Doff WAS; Wever PC; Thai KTD; van Kampen JJA; Haagmans BL; Demmers JAA PLoS One 2021[]; 16 (11): e0259165 PMID34762662show ga The rapid, sensitive and specific detection of SARS-CoV-2 is critical in responding to the current COVID-19 outbreak. In this proof-of-concept study, we explored the potential of targeted mass spectrometry (MS) based proteomics for the detection of SARS-CoV-2 proteins in both research samples and clinical specimens. First, we assessed the limit of detection for several SARS-CoV-2 proteins by parallel reaction monitoring (PRM) MS in infected Vero E6 cells. For tryptic peptides of Nucleocapsid protein, the limit of detection was estimated to be in the mid-attomole range (9E-13 g). Next, this PRM methodology was applied to the detection of viral proteins in various COVID-19 patient clinical specimens, such as sputum and nasopharyngeal swabs. SARS-CoV-2 proteins were detected in these samples with high sensitivity in all specimens with PCR Ct values <24 and in several samples with higher CT values. A clear relationship was observed between summed MS peak intensities for SARS-CoV-2 proteins and Ct values reflecting the abundance of viral RNA. Taken together, these results suggest that targeted MS based proteomics may have the potential to be used as an additional tool in COVID-19 diagnostics. |*Proteomics[MESH] |Animals[MESH] |COVID-19/*diagnosis/pathology/virology[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Mass Spectrometry[MESH] |Nucleocapsid/genetics/isolation & purification[MESH] |Phosphoproteins/genetics/isolation & purification[MESH] |Proteome/genetics[MESH] |SARS-CoV-2/genetics/*isolation & purification/pathogenicity[MESH] |Sputum/virology[MESH] |Vero Cells[MESH]Targeted proteomics as a tool to detect SARS-CoV-2 proteins in clinica(epmc).pdf DeepDyve Pubget Overpricing