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10.1038/s41592-021-01317-x http://scihub22266oqcxt.onion/10.1038/s41592-021-01317-x 34737453!8648561!34737453     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Methods 2021 ; 18 (12): 1532-1541 Nephropedia Template TP {{tp|p=34737453|t=2021. Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography |pdf=|usr=}}{{34737453}} gab.com Text Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography JO: Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=34737453 #FOAvip Imaging intact human organs from the organ to the cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using the European Synchrotron Radiation Facility (ESRF)'s Extremely Brilliant Source (EBS). The spatial coherence of the ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us to perform non-destructive, three-dimensional (3D) scans with hierarchically increasing resolution at any location in whole human organs. We applied HiP-CT to image five intact human organ types: brain, lung, heart, kidney and spleen. HiP-CT provided a structural overview of each whole organ followed by multiple higher-resolution volumes of interest, capturing organotypic functional units and certain individual specialized cells within intact human organs. We demonstrate the potential applications of HiP-CT through quantification and morphometry of glomeruli in an intact human kidney and identification of regional changes in the tissue architecture in a lung from a deceased donor with coronavirus disease 2019 (COVID-19). Twit Text FOAVip Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography ????Nat Methods http://www.kidney.de/pget.php?&tw=1&pmid=34737453 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34737453 #FOAvip English Wikipedia <ref>{{Cite pmid|34737453}}</ref> Imaging intact human organs with local resolution of cellular structures using hierarchical phase-contrast tomography #MMPMID34737453 Walsh CL; Tafforeau P; Wagner WL; Jafree DJ; Bellier A; Werlein C; Kuhnel MP; Boller E; Walker-Samuel S; Robertus JL; Long DA; Jacob J; Marussi S; Brown E; Holroyd N; Jonigk DD; Ackermann M; Lee PD Nat Methods 2021[Dec]; 18 (12): 1532-1541 PMID34737453show ga Imaging intact human organs from the organ to the cellular scale in three dimensions is a goal of biomedical imaging. To meet this challenge, we developed hierarchical phase-contrast tomography (HiP-CT), an X-ray phase propagation technique using the European Synchrotron Radiation Facility (ESRF)'s Extremely Brilliant Source (EBS). The spatial coherence of the ESRF-EBS combined with our beamline equipment, sample preparation and scanning developments enabled us to perform non-destructive, three-dimensional (3D) scans with hierarchically increasing resolution at any location in whole human organs. We applied HiP-CT to image five intact human organ types: brain, lung, heart, kidney and spleen. HiP-CT provided a structural overview of each whole organ followed by multiple higher-resolution volumes of interest, capturing organotypic functional units and certain individual specialized cells within intact human organs. We demonstrate the potential applications of HiP-CT through quantification and morphometry of glomeruli in an intact human kidney and identification of regional changes in the tissue architecture in a lung from a deceased donor with coronavirus disease 2019 (COVID-19). |*SARS-CoV-2[MESH] |COVID-19/*pathology[MESH] |Humans[MESH] |Image Processing, Computer-Assisted/*methods[MESH] |Imaging, Three-Dimensional/*methods[MESH] |Kidney/anatomy & histology[MESH] |Lung/*pathology[MESH] |Synchrotrons[MESH]Imaging intact human organs with local resolution of cellular structur(epmc).pdf DeepDyve Pubget Overpricing