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Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell+Rep+Methods 2021 ; 1 (7): 100117 Nephropedia Template TP
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Using soft X-ray tomography for rapid whole-cell quantitative imaging of SARS-CoV-2-infected cells #MMPMID34729550
Loconte V; Chen JH; Cortese M; Ekman A; Le Gros MA; Larabell C; Bartenschlager R; Weinhardt V
Cell Rep Methods 2021[Nov]; 1 (7): 100117 PMID34729550show ga
High-resolution and rapid imaging of host cell ultrastructure can generate insights toward viral disease mechanism, for example for a severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. Here, we employ full-rotation soft X-ray tomography (SXT) to examine organelle remodeling induced by SARS-CoV-2 at the whole-cell level with high spatial resolution and throughput. Most of the current SXT systems suffer from a restricted field of view due to use of flat sample supports and artifacts due to missing data. In this approach using cylindrical sample holders, a full-rotation tomogram of human lung epithelial cells is performed in less than 10 min. We demonstrate the potential of SXT imaging by visualizing aggregates of SARS-CoV-2 virions and virus-induced intracellular alterations. This rapid whole-cell imaging approach allows us to visualize the spatiotemporal changes of cellular organelles upon viral infection in a quantitative manner.