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10.1073/pnas.2111815118 http://scihub22266oqcxt.onion/10.1073/pnas.2111815118 34725257!8609653!34725257     free     free     free Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 227.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (46): ä Nephropedia Template TP {{tp|p=34725257|t=2021. Mass spectrometric identification of immunogenic SARS-CoV-2 epitopes and cognate TCRs |pdf=|usr=}}{{34725257}} gab.com Text Mass spectrometric identification of immunogenic SARS-CoV-2 epitopes and cognate TCRs JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34725257 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections elicit both humoral and cellular immune responses. For the prevention and treatment of COVID-19, the disease caused by SARS-CoV-2, it has become increasingly apparent that T cell responses are equally if not more important than humoral responses in mediating recovery and immune protection. One major challenge in developing T cell-based therapies for infectious and malignant diseases has been the identification of immunogenic epitopes that can elicit a meaningful T cell response. Traditionally, this has been achieved using sophisticated in silico methods to predict putative epitopes deduced from binding affinities. Our studies find that, in contrast to current convention, "immunodominant" SARS-CoV-2 peptides defined by such in silico methods often fail to elicit T cell responses recognizing naturally presented SARS-CoV-2 epitopes. We postulated that immunogenic epitopes for SARS-CoV-2 are best defined empirically by directly analyzing peptides eluted from the naturally processed peptide-major histocompatibility complex (MHC) and then validating immunogenicity by determining whether such peptides can elicit T cells recognizing SARS-CoV-2 antigen-expressing cells. Using a tandem mass spectrometry approach, we identified epitopes derived from not only structural but also nonstructural genes in regions highly conserved among SARS-CoV-2 strains, including recently recognized variants. Finally, there are no reported T cell receptor-engineered T cell technology that can redirect T cell specificity to recognize and kill SARS-CoV-2 target cells. We report here several SARS-CoV-2 epitopes defined by mass spectrometric analysis of MHC-eluted peptides, provide empiric evidence for their immunogenicity, and demonstrate engineered TCR-redirected killing. Twit Text FOAVip Mass spectrometric identification of immunogenic SARS-CoV-2 epitopes and cognate TCRs ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34725257 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34725257 #FOAvip English Wikipedia <ref>{{Cite pmid|34725257}}</ref> Mass spectrometric identification of immunogenic SARS-CoV-2 epitopes and cognate TCRs #MMPMID34725257 Pan K; Chiu Y; Huang E; Chen M; Wang J; Lai I; Singh S; Shaw RM; MacCoss MJ; Yee C Proc Natl Acad Sci U S A 2021[Nov]; 118 (46): ä PMID34725257show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections elicit both humoral and cellular immune responses. For the prevention and treatment of COVID-19, the disease caused by SARS-CoV-2, it has become increasingly apparent that T cell responses are equally if not more important than humoral responses in mediating recovery and immune protection. One major challenge in developing T cell-based therapies for infectious and malignant diseases has been the identification of immunogenic epitopes that can elicit a meaningful T cell response. Traditionally, this has been achieved using sophisticated in silico methods to predict putative epitopes deduced from binding affinities. Our studies find that, in contrast to current convention, "immunodominant" SARS-CoV-2 peptides defined by such in silico methods often fail to elicit T cell responses recognizing naturally presented SARS-CoV-2 epitopes. We postulated that immunogenic epitopes for SARS-CoV-2 are best defined empirically by directly analyzing peptides eluted from the naturally processed peptide-major histocompatibility complex (MHC) and then validating immunogenicity by determining whether such peptides can elicit T cells recognizing SARS-CoV-2 antigen-expressing cells. Using a tandem mass spectrometry approach, we identified epitopes derived from not only structural but also nonstructural genes in regions highly conserved among SARS-CoV-2 strains, including recently recognized variants. Finally, there are no reported T cell receptor-engineered T cell technology that can redirect T cell specificity to recognize and kill SARS-CoV-2 target cells. We report here several SARS-CoV-2 epitopes defined by mass spectrometric analysis of MHC-eluted peptides, provide empiric evidence for their immunogenicity, and demonstrate engineered TCR-redirected killing. |*SARS-CoV-2[MESH] |CD8-Positive T-Lymphocytes[MESH] |COVID-19/*immunology[MESH] |Cell Line[MESH] |Epitopes, T-Lymphocyte/immunology/*isolation & purification[MESH] |Epitopes/genetics/*isolation & purification[MESH] |Humans[MESH] |Major Histocompatibility Complex[MESH] |Mass Spectrometry/*methods[MESH] |Peptides[MESH] |Receptors, Antigen, T-Cell/genetics/*immunology[MESH]Mass spectrometric identification of immunogenic SARS-CoV-2 epitopes a(epmc).pdf DeepDyve Pubget Overpricing