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10.7883/yoken.JJID.2021.292 http://scihub22266oqcxt.onion/10.7883/yoken.JJID.2021.292 34719530!ä!34719530 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Jpn+J+Infect+Dis 2022 ; 75 (3): 277-280 Nephropedia Template TP {{tp|p=34719530|t=2022. Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Individual |pdf=|usr=}}{{34719530}} gab.com Text Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Individual JO: Jpn J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34719530 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, in December 2019. Despite the recent introduction of vaccines against SARS-CoV-2, more effective vaccines and antiviral drugs must be developed. Here, we isolated five SARS-CoV-2 strains from four patients with coronavirus disease (COVID-19) and an asymptomatic individual using pharyngeal swabs, nasopharyngeal swabs, and sputum samples. Cytopathic effects in inoculated Vero cells were observed between days 3 and 7. SARS-CoV-2 infection was confirmed by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) and next-generation sequencing. Phylogenetic analyses of the whole genome sequences showed that the virus isolates from the clinical samples belonged to the Wuhan and European lineages. These findings and the isolated viruses may contribute to the development of diagnostic tools, vaccines, and antiviral drugs for COVID-19. Twit Text FOAVip Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Individual ????Jpn J Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34719530 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34719530 #FOAvip English Wikipedia <ref>{{Cite pmid|34719530}}</ref> Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Individual #MMPMID34719530 Hishiki T; Usui K; An T; Suzuki R; Sakuragi JI; Tanaka Y; Matsuki Y; Kawai J; Kogo Y; Hayashizaki Y; Takasaki T Jpn J Infect Dis 2022[May]; 75 (3): 277-280 PMID34719530show ga Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) emerged in Wuhan, China, in December 2019. Despite the recent introduction of vaccines against SARS-CoV-2, more effective vaccines and antiviral drugs must be developed. Here, we isolated five SARS-CoV-2 strains from four patients with coronavirus disease (COVID-19) and an asymptomatic individual using pharyngeal swabs, nasopharyngeal swabs, and sputum samples. Cytopathic effects in inoculated Vero cells were observed between days 3 and 7. SARS-CoV-2 infection was confirmed by quantitative reverse-transcription polymerase chain reaction (RT-qPCR) and next-generation sequencing. Phylogenetic analyses of the whole genome sequences showed that the virus isolates from the clinical samples belonged to the Wuhan and European lineages. These findings and the isolated viruses may contribute to the development of diagnostic tools, vaccines, and antiviral drugs for COVID-19. |*COVID-19[MESH] |*SARS-CoV-2/genetics[MESH] |Animals[MESH] |Antiviral Agents/therapeutic use[MESH] |COVID-19 Vaccines[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Phylogeny[MESH]Isolation of SARS-CoV-2 from COVID-19 Patients and an Asymptomatic Ind(epmc).pdf DeepDyve Pubget Overpricing