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10.1073/pnas.2105253118 http://scihub22266oqcxt.onion/10.1073/pnas.2105253118 34716263!8612357!34716263     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Proc+Natl+Acad+Sci+U+S+A 2021 ; 118 (44): ä Nephropedia Template TP {{tp|p=34716263|t=2021. SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection |pdf=|usr=}}{{34716263}} gab.com Text SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection JO: Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34716263 #FOAvip SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations of cats, mink, and ferrets have perpetuated transmission cycles. We sequenced full genomes of Vero cell-expanded SARS-CoV-2 inoculum and viruses recovered from cats (n = 6), dogs (n = 3), hamsters (n = 3), and a ferret (n = 1) following experimental exposure. Five nonsynonymous changes relative to the USA-WA1/2020 prototype strain were near fixation in the stock used for inoculation but had reverted to wild-type sequences at these sites in dogs, cats, and hamsters within 1- to 3-d postexposure. A total of 14 emergent variants (six in nonstructural genes, six in spike, and one each in orf8 and nucleocapsid) were detected in viruses recovered from animals. This included substitutions in spike residues H69, N501, and D614, which also vary in human lineages of concern. Even though a live virus was not cultured from dogs, substitutions in replicase genes were detected in amplified sequences. The rapid selection of SARS-CoV-2 variants in vitro and in vivo reveals residues with functional significance during host switching. These observations also illustrate the potential for spillback from animal hosts to accelerate the evolution of new viral lineages, findings of particular concern for dogs and cats living in households with COVID-19 patients. More generally, this glimpse into viral host switching reveals the unrealized rapidity and plasticity of viral evolution in experimental animal model systems. Twit Text FOAVip SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection ????Proc Natl Acad Sci U S A http://www.kidney.de/pget.php?&tw=1&pmid=34716263 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34716263 #FOAvip English Wikipedia <ref>{{Cite pmid|34716263}}</ref> SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant selection #MMPMID34716263 Bashor L; Gagne RB; Bosco-Lauth AM; Bowen RA; Stenglein M; VandeWoude S Proc Natl Acad Sci U S A 2021[Nov]; 118 (44): ä PMID34716263show ga SARS-CoV-2 spillback from humans into domestic and wild animals has been well documented, and an accumulating number of studies illustrate that human-to-animal transmission is widespread in cats, mink, deer, and other species. Experimental inoculations of cats, mink, and ferrets have perpetuated transmission cycles. We sequenced full genomes of Vero cell-expanded SARS-CoV-2 inoculum and viruses recovered from cats (n = 6), dogs (n = 3), hamsters (n = 3), and a ferret (n = 1) following experimental exposure. Five nonsynonymous changes relative to the USA-WA1/2020 prototype strain were near fixation in the stock used for inoculation but had reverted to wild-type sequences at these sites in dogs, cats, and hamsters within 1- to 3-d postexposure. A total of 14 emergent variants (six in nonstructural genes, six in spike, and one each in orf8 and nucleocapsid) were detected in viruses recovered from animals. This included substitutions in spike residues H69, N501, and D614, which also vary in human lineages of concern. Even though a live virus was not cultured from dogs, substitutions in replicase genes were detected in amplified sequences. The rapid selection of SARS-CoV-2 variants in vitro and in vivo reveals residues with functional significance during host switching. These observations also illustrate the potential for spillback from animal hosts to accelerate the evolution of new viral lineages, findings of particular concern for dogs and cats living in households with COVID-19 patients. More generally, this glimpse into viral host switching reveals the unrealized rapidity and plasticity of viral evolution in experimental animal model systems. |*Evolution, Molecular[MESH] |*Selection, Genetic[MESH] |Animals[MESH] |COVID-19/veterinary/*virology[MESH] |Cats[MESH] |Chlorocebus aethiops[MESH] |Dogs[MESH] |Ferrets[MESH] |Gene Frequency[MESH] |Pets/virology[MESH] |SARS-CoV-2/*genetics/pathogenicity[MESH] |Vero Cells[MESH]SARS-CoV-2 evolution in animals suggests mechanisms for rapid variant (epmc).pdf DeepDyve Pubget Overpricing