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10.1371/journal.pone.0259277 http://scihub22266oqcxt.onion/10.1371/journal.pone.0259277 34714886!8555800!34714886     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34714886.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2021 ; 16 (10): e0259277 Nephropedia Template TP {{tp|p=34714886|t=2021. Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols |pdf=|usr=}}{{34714886}} gab.com Text Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34714886 #FOAvip Surveillance of the SARS-CoV-2 variants including the quickly spreading mutants by rapid and near real-time sequencing of the viral genome provides an important tool for effective health policy decision making in the ongoing COVID-19 pandemic. Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the SARS-CoV-2 genome sequences. Analysis of several sequencing runs demonstrated that using the long amplicon schemes outperforms the original protocol based on the 400-bp amplicons. It also illustrated common artefacts and problems associated with PCR-tiling approach, such as uneven genome coverage, variable fraction of discarded sequencing reads, including human and bacterial contamination, as well as the presence of reads derived from the viral sub-genomic RNAs. Twit Text FOAVip Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34714886 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34714886 #FOAvip English Wikipedia <ref>{{Cite pmid|34714886}}</ref> Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-tiling amplicon protocols #MMPMID34714886 Brejova B; Borsova K; Hodorova V; Cabanova V; Gafurov A; Fricova D; Nebohacova M; Vinar T; Klempa B; Nosek J PLoS One 2021[]; 16 (10): e0259277 PMID34714886show ga Surveillance of the SARS-CoV-2 variants including the quickly spreading mutants by rapid and near real-time sequencing of the viral genome provides an important tool for effective health policy decision making in the ongoing COVID-19 pandemic. Here we evaluated PCR-tiling of short (~400-bp) and long (~2 and ~2.5-kb) amplicons combined with nanopore sequencing on a MinION device for analysis of the SARS-CoV-2 genome sequences. Analysis of several sequencing runs demonstrated that using the long amplicon schemes outperforms the original protocol based on the 400-bp amplicons. It also illustrated common artefacts and problems associated with PCR-tiling approach, such as uneven genome coverage, variable fraction of discarded sequencing reads, including human and bacterial contamination, as well as the presence of reads derived from the viral sub-genomic RNAs. |*Pandemics[MESH] |*SARS-CoV-2/genetics/isolation & purification[MESH] |COVID-19/*diagnosis[MESH] |Humans[MESH]Nanopore sequencing of SARS-CoV-2: Comparison of short and long PCR-ti(epmc).pdf DeepDyve Pubget Overpricing