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10.1038/s42003-021-02748-0 http://scihub22266oqcxt.onion/10.1038/s42003-021-02748-0 34686777!8536764!34686777     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34686777&cmd=llinks): Failed to open stream: HTTP request failed! 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Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis |pdf=|usr=}}{{34686777}} gab.com Text Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis JO: Commun Biol http://www.kidney.de/pget.php?&tw=1&pmid=34686777 #FOAvip SARS-CoV-2 replication requires the synthesis of a set of structural proteins expressed through discontinuous transcription of ten subgenomic mRNAs (sgmRNAs). Here, we have fine-tuned droplet digital PCR (ddPCR) assays to accurately detect and quantify SARS-CoV-2 genomic ORF1ab and sgmRNAs for the nucleocapsid (N) and spike (S) proteins. We analyzed 166 RNA samples from anonymized SARS-CoV-2 positive subjects and we observed a recurrent and characteristic pattern of sgmRNAs expression in relation to the total viral RNA content. Additionally, expression profiles of sgmRNAs, as determined by meta-transcriptomics sequencing of a subset of 110 RNA samples, were highly correlated with those obtained by ddPCR. By providing a comprehensive and dynamic snapshot of the levels of SARS-CoV-2 sgmRNAs in infected individuals, our results may contribute a better understanding of the dynamics of transcription and expression of the genome of SARS-CoV-2 and facilitate the development of more accurate molecular diagnostic tools for the stratification of COVID-19 patients. Twit Text FOAVip Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis ????Commun Biol http://www.kidney.de/pget.php?&tw=1&pmid=34686777 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34686777 #FOAvip English Wikipedia <ref>{{Cite pmid|34686777}}</ref> Accurate detection and quantification of SARS-CoV-2 genomic and subgenomic mRNAs by ddPCR and meta-transcriptomics analysis #MMPMID34686777 Oranger A; Manzari C; Chiara M; Notario E; Fosso B; Parisi A; Bianco A; Iacobellis M; d'Avenia M; D'Erchia AM; Pesole G Commun Biol 2021[Oct]; 4 (1): 1215 PMID34686777show ga SARS-CoV-2 replication requires the synthesis of a set of structural proteins expressed through discontinuous transcription of ten subgenomic mRNAs (sgmRNAs). Here, we have fine-tuned droplet digital PCR (ddPCR) assays to accurately detect and quantify SARS-CoV-2 genomic ORF1ab and sgmRNAs for the nucleocapsid (N) and spike (S) proteins. We analyzed 166 RNA samples from anonymized SARS-CoV-2 positive subjects and we observed a recurrent and characteristic pattern of sgmRNAs expression in relation to the total viral RNA content. Additionally, expression profiles of sgmRNAs, as determined by meta-transcriptomics sequencing of a subset of 110 RNA samples, were highly correlated with those obtained by ddPCR. By providing a comprehensive and dynamic snapshot of the levels of SARS-CoV-2 sgmRNAs in infected individuals, our results may contribute a better understanding of the dynamics of transcription and expression of the genome of SARS-CoV-2 and facilitate the development of more accurate molecular diagnostic tools for the stratification of COVID-19 patients. |*Coronavirus Nucleocapsid Proteins[MESH] |*SARS-CoV-2[MESH] |*Spike Glycoprotein, Coronavirus[MESH] |*Transcriptome[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |COVID-19/*genetics/*metabolism[MESH] |Computational Biology[MESH] |Humans[MESH] |Limit of Detection[MESH] |Open Reading Frames[MESH] |Phosphoproteins[MESH] |Polymerase Chain Reaction/*methods[MESH] |RNA, Messenger/metabolism[MESH] |RNA, Viral/*metabolism[MESH] |Real-Time Polymerase Chain Reaction[MESH]Accurate detection and quantification of SARS-CoV-2 genomic and subgen(epmc).pdf DeepDyve Pubget Overpricing