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10.1038/s41587-021-01065-5 http://scihub22266oqcxt.onion/10.1038/s41587-021-01065-5 34675424!10549956!34675424     free     free     free Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34675424.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nat+Biotechnol 2022 ; 40 (3): 374-381 Nephropedia Template TP {{tp|p=34675424|t=2022. Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC |pdf=|usr=}}{{34675424}} gab.com Text Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC JO: Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34675424 #FOAvip Multimodal measurements of single-cell profiles are proving increasingly useful for characterizing cell states and regulatory mechanisms. In the present study, we developed PHAGE-ATAC (Assay for Transposase-Accessible Chromatin), a massively parallel droplet-based method that uses phage displaying, engineered, camelid single-domain antibodies ('nanobodies') for simultaneous single-cell measurements of protein levels and chromatin accessibility profiles, and mitochondrial DNA-based clonal tracing. We use PHAGE-ATAC for multimodal analysis in primary human immune cells, sample multiplexing, intracellular protein analysis and the detection of SARS-CoV-2 spike protein in human cell populations. Finally, we construct a synthetic high-complexity phage library for selection of antigen-specific nanobodies that bind cells of particular molecular profiles, opening an avenue for protein detection, cell characterization and screening with single-cell genomics. Twit Text FOAVip Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC ????Nat Biotechnol http://www.kidney.de/pget.php?&tw=1&pmid=34675424 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34675424 #FOAvip English Wikipedia <ref>{{Cite pmid|34675424}}</ref> Single-cell profiling of proteins and chromatin accessibility using PHAGE-ATAC #MMPMID34675424 Fiskin E; Lareau CA; Ludwig LS; Eraslan G; Liu F; Ring AM; Xavier RJ; Regev A Nat Biotechnol 2022[Mar]; 40 (3): 374-381 PMID34675424show ga Multimodal measurements of single-cell profiles are proving increasingly useful for characterizing cell states and regulatory mechanisms. In the present study, we developed PHAGE-ATAC (Assay for Transposase-Accessible Chromatin), a massively parallel droplet-based method that uses phage displaying, engineered, camelid single-domain antibodies ('nanobodies') for simultaneous single-cell measurements of protein levels and chromatin accessibility profiles, and mitochondrial DNA-based clonal tracing. We use PHAGE-ATAC for multimodal analysis in primary human immune cells, sample multiplexing, intracellular protein analysis and the detection of SARS-CoV-2 spike protein in human cell populations. Finally, we construct a synthetic high-complexity phage library for selection of antigen-specific nanobodies that bind cells of particular molecular profiles, opening an avenue for protein detection, cell characterization and screening with single-cell genomics. |*Bacteriophages/genetics[MESH] |*COVID-19[MESH] |Chromatin/genetics[MESH] |Humans[MESH] |SARS-CoV-2[MESH] |Single-Cell Analysis/methods[MESH]Single-cell profiling of proteins and chromatin accessibility using PH(epmc).pdf DeepDyve Pubget Overpricing