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Deprecated: Implicit conversion from float 267.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Cell+Chem+Biol 2022 ; 29 (1): 5-18.e6 Nephropedia Template TP
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An antibody-based proximity labeling map reveals mechanisms of SARS-CoV-2 inhibition of antiviral immunity #MMPMID34672954
Zhang Y; Shang L; Zhang J; Liu Y; Jin C; Zhao Y; Lei X; Wang W; Xiao X; Zhang X; Liu Y; Liu L; Zhuang MW; Mi Q; Tian C; Wang J; He F; Wang PH; Wang J
Cell Chem Biol 2022[Jan]; 29 (1): 5-18.e6 PMID34672954show ga
The global epidemic caused by the coronavirus severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) has resulted in the infection of over 200 million people. To extend the knowledge of interactions between SARS-CoV-2 and humans, we systematically investigate the interactome of 29 viral proteins in human cells by using an antibody-based TurboID assay. In total, 1,388 high-confidence human proximal proteins with biotinylated sites are identified. Notably, we find that SARS-CoV-2 manipulates the antiviral and immune responses. We validate that the membrane protein ITGB1 associates angiotensin-converting enzyme 2 (ACE2) to mediate SARS-CoV-2 entry. Moreover, we reveal that SARS-CoV-2 proteins inhibit activation of the interferon pathway through the mitochondrial protein mitochondrial antiviral-signaling protein (MAVS) and the methyltransferase SET domain containing 2, histone lysine methyltransferase (SETD2). We propose 111 potential drugs for the clinical treatment of coronavirus disease 2019 (COVID-19) and identify three compounds that significantly inhibit the replication of SARS-CoV-2. The proximity labeling map of SARS-CoV-2 and humans provides a resource for elucidating the mechanisms of viral infection and developing drugs for COVID-19 treatment.