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10.1128/JCM.01609-21

http://scihub22266oqcxt.onion/10.1128/JCM.01609-21
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suck abstract from ncbi


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pmid34669457      J+Clin+Microbiol 2022 ; 60 (1): e0160921
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  • Viral Culture Confirmed SARS-CoV-2 Subgenomic RNA Value as a Good Surrogate Marker of Infectivity #MMPMID34669457
  • Santos Bravo M; Berengua C; Marin P; Esteban M; Rodriguez C; Del Cuerpo M; Miro E; Cuesta G; Mosquera M; Sanchez-Palomino S; Vila J; Rabella N; Marcos MA
  • J Clin Microbiol 2022[Jan]; 60 (1): e0160921 PMID34669457show ga
  • Determining SARS-CoV-2 viral infectivity is crucial for patient clinical assessment and isolation decisions. We assessed subgenomic RNA (sgRNA) as a surrogate marker of SARS-CoV-2 infectivity in SARS-CoV-2-positive reverse transcription PCR (RT-PCR) respiratory samples (n = 105) in comparison with viral culture as the reference standard for virus replication. sgRNA and viral isolation results were concordant in 99/105 cases (94%), indicating highly significant agreement between the two techniques (Cohen's kappa coefficient 0.88, 95% confidence interval [CI] 0.78 to 0.97, P < 0.001). sgRNA RT-PCR showed a sensitivity of 97% and a positive predictive value of 94% to detect replication-competent virus, further supporting sgRNA as a surrogate marker of SARS-CoV-2 infectivity. sgRNA RT-PCR is an accurate, rapid, and affordable technique that can overcome culture and cycle threshold (C(T)) value limitations and be routinely implemented in hospital laboratories to detect viral infectivity, which is essential for optimizing patient monitoring, the efficacy of treatments/vaccines, and work reincorporation policies, as well as for safely shortening isolation precautions.
  • |*COVID-19[MESH]
  • |*SARS-CoV-2[MESH]
  • |Biomarkers[MESH]
  • |Humans[MESH]
  • |RNA[MESH]
  • |RNA, Viral/genetics[MESH]


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