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10.1016/j.medcli.2021.09.013

http://scihub22266oqcxt.onion/10.1016/j.medcli.2021.09.013
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suck abstract from ncbi


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pmid34666900      Med+Clin+(Barc) 2022 ; 159 (3): 116-123
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  • Commercialized kits to assess T-cell responses against SARS-CoV-2 S peptides A pilot study in health care workers #MMPMID34666900
  • Martinez-Gallo M; Esperalba J; Pujol-Borrell R; Sanda V; Arrese-Munoz I; Fernandez-Naval C; Anton A; Cardona V; Labrador-Horrillo M; Pumarola T; Hernandez-Gonzalez M
  • Med Clin (Barc) 2022[Aug]; 159 (3): 116-123 PMID34666900show ga
  • BACKGROUND: It is crucial to assess the levels of protection generated by natural infection or SARS-CoV-2 vaccines, mainly in individuals professionally exposed and in vulnerable groups. Measuring T-cell responses may complement antibody tests currently in use as correlates of protection. Our aim was to assess the feasibility of a validated assay of T-cell responses. METHODS: Twenty health-care-workers (HCW) were included. Antibody test to SARS-CoV-2 N and S-proteins in parallel with a commercially available whole-blood-interferon-gamma-release-assay (IGRA) to S-peptides and two detection methods, CLIA and ELISA were determined. RESULTS: IGRA test detected T-cell responses in naturally exposed and vaccinated HCW already after first vaccination dose. The correlation by the two detection methods was very high (R>0.8) and sensitivity and specificity ranged between 100 and 86% and 100-73% respectively. Even though there was a very high concordance between specific antibody levels and the IGRA assay in the ability to detect immune response to SARS-CoV-2, there was a relatively low quantitative correlation. In the small group primed by natural infection, one vaccine dose was sufficient to reach immune response plateau. IGRA was positive in one, with Ig(S) antibody negative vaccinated immunosuppressed HCW illustrating another advantage of the IGRA-test. CONCLUSION: Whole-blood-IGRA-tests amenable to automation and constitutes a promising additional tool for measuring the state of the immune response to SARS-CoV-2; they are applicable to large number of samples and may become a valuable correlate of protection to COVID-19, particularly for vulnerable groups at risk of being re-exposed to infection, as are health-care-workers.
  • |*COVID-19[MESH]
  • |*SARS-CoV-2[MESH]
  • |Antibodies, Viral[MESH]
  • |COVID-19 Vaccines[MESH]
  • |Health Personnel[MESH]
  • |Humans[MESH]
  • |Peptides[MESH]
  • |Pilot Projects[MESH]


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