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10.1126/science.abj8430 http://scihub22266oqcxt.onion/10.1126/science.abj8430 34648371!9007107!34648371     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Science 2021 ; 374 (6571): 1099-1106 Nephropedia Template TP {{tp|p=34648371|t=2021. Replication and single-cycle delivery of SARS-CoV-2 replicons |pdf=|usr=}}{{34648371}} gab.com Text Replication and single-cycle delivery of SARS-CoV-2 replicons JO: Science http://www.kidney.de/pget.php?&tw=1&pmid=34648371 #FOAvip Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization. Twit Text FOAVip Replication and single-cycle delivery of SARS-CoV-2 replicons ????Science http://www.kidney.de/pget.php?&tw=1&pmid=34648371 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34648371 #FOAvip English Wikipedia <ref>{{Cite pmid|34648371}}</ref> Replication and single-cycle delivery of SARS-CoV-2 replicons #MMPMID34648371 Ricardo-Lax I; Luna JM; Thao TTN; Le Pen J; Yu Y; Hoffmann HH; Schneider WM; Razooky BS; Fernandez-Martinez J; Schmidt F; Weisblum Y; Trueb BS; Berenguer Veiga I; Schmied K; Ebert N; Michailidis E; Peace A; Sanchez-Rivera FJ; Lowe SW; Rout MP; Hatziioannou T; Bieniasz PD; Poirier JT; MacDonald MR; Thiel V; Rice CM Science 2021[Nov]; 374 (6571): 1099-1106 PMID34648371show ga Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization. |Animals[MESH] |Antibodies, Neutralizing/immunology[MESH] |Antibodies, Viral/immunology[MESH] |Antiviral Agents/pharmacology[MESH] |Cell Line[MESH] |Humans[MESH] |Interferons/pharmacology[MESH] |Microbial Sensitivity Tests[MESH] |Mutation[MESH] |Plasmids[MESH] |RNA, Viral/*genetics/metabolism[MESH] |Replicon/genetics/*physiology[MESH] |Reverse Genetics[MESH] |SARS-CoV-2/drug effects/*genetics/physiology[MESH] |Saccharomyces cerevisiae/genetics[MESH] |Spike Glycoprotein, Coronavirus/genetics[MESH] |Viral Nonstructural Proteins/genetics/metabolism[MESH] |Viral Pseudotyping[MESH] |Virion/genetics/physiology[MESH]Replication and single-cycle delivery of SARS-CoV-2 replicons (epmc).pdf DeepDyve Pubget Overpricing