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10.1126/sciimmunol.abl5842 http://scihub22266oqcxt.onion/10.1126/sciimmunol.abl5842 34648356!8720485!34648356     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34648356&cmd=llinks): Failed to open stream: HTTP request failed! 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Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses |pdf=|usr=}}{{34648356}} gab.com Text Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses JO: Sci Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34648356 #FOAvip Initial exposure to a pathogen elicits an adaptive immune response to control and eradicate the threat. Interrogating the abundance and specificity of the naive B cell repertoire drives understanding of how to mount protective responses. Here, we isolated naive B cells from eight seronegative human donors targeting the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD). Single-cell B cell receptor (BCR) sequencing identified diverse gene usage and no restriction on complementarity determining region length. A subset of recombinant antibodies produced by naive B cell precursors bound to SARS-CoV-2 RBD and engaged circulating variants including B.1.1.7, B.1.351, and B.1.617.2, as well as preemergent bat-derived coronaviruses RaTG13, SHC104, and WIV1. By structural characterization of a naive antibody in complex with SARS-CoV-2 spike, we identified a conserved mode of recognition shared with infection-induced antibodies. We found that representative naive antibodies could signal in a B cell activation assay, and by using directed evolution, we could select for a higher-affinity RBD interaction, conferred by a single amino acid change. The minimally mutated, affinity-matured antibodies also potently neutralized SARS-CoV-2. Understanding the SARS-CoV-2 RBD-specific naive repertoire may inform potential responses capable of recognizing future SARS-CoV-2 variants or emerging coronaviruses, enabling the development of pan-coronavirus vaccines aimed at engaging protective germline responses. Twit Text FOAVip Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses ????Sci Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34648356 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34648356 #FOAvip English Wikipedia <ref>{{Cite pmid|34648356}}</ref> Naive human B cells engage the receptor binding domain of SARS-CoV-2, variants of concern, and related sarbecoviruses #MMPMID34648356 Feldman J; Bals J; Altomare CG; St Denis K; Lam EC; Hauser BM; Ronsard L; Sangesland M; Moreno TB; Okonkwo V; Hartojo N; Balazs AB; Bajic G; Lingwood D; Schmidt AG Sci Immunol 2021[Dec]; 6 (66): eabl5842 PMID34648356show ga Initial exposure to a pathogen elicits an adaptive immune response to control and eradicate the threat. Interrogating the abundance and specificity of the naive B cell repertoire drives understanding of how to mount protective responses. Here, we isolated naive B cells from eight seronegative human donors targeting the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) receptor binding domain (RBD). Single-cell B cell receptor (BCR) sequencing identified diverse gene usage and no restriction on complementarity determining region length. A subset of recombinant antibodies produced by naive B cell precursors bound to SARS-CoV-2 RBD and engaged circulating variants including B.1.1.7, B.1.351, and B.1.617.2, as well as preemergent bat-derived coronaviruses RaTG13, SHC104, and WIV1. By structural characterization of a naive antibody in complex with SARS-CoV-2 spike, we identified a conserved mode of recognition shared with infection-induced antibodies. We found that representative naive antibodies could signal in a B cell activation assay, and by using directed evolution, we could select for a higher-affinity RBD interaction, conferred by a single amino acid change. The minimally mutated, affinity-matured antibodies also potently neutralized SARS-CoV-2. Understanding the SARS-CoV-2 RBD-specific naive repertoire may inform potential responses capable of recognizing future SARS-CoV-2 variants or emerging coronaviruses, enabling the development of pan-coronavirus vaccines aimed at engaging protective germline responses. |Antibodies, Neutralizing/immunology[MESH] |Antigens, Viral/immunology[MESH] |B-Lymphocytes/*cytology/*immunology/metabolism[MESH] |COVID-19 Vaccines/immunology[MESH] |COVID-19/immunology[MESH] |Coronavirus/*immunology[MESH] |Epitopes[MESH] |Humans[MESH] |Lymphocyte Activation[MESH] |SARS-CoV-2/classification/*immunology/metabolism[MESH]Naive human B cells engage the receptor binding domain of SARS-CoV-2, (epmc).pdf DeepDyve Pubget Overpricing