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10.1371/journal.pbio.3001425 http://scihub22266oqcxt.onion/10.1371/journal.pbio.3001425 34634033!8553124!34634033     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       PLoS+Biol 2021 ; 19 (10): e3001425 Nephropedia Template TP {{tp|p=34634033|t=2021. SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA |pdf=|usr=}}{{34634033}} gab.com Text SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA JO: PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=34634033 #FOAvip The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection causes Coronavirus Disease 2019 (COVID-19), a pandemic that seriously threatens global health. SARS-CoV-2 propagates by packaging its RNA genome into membrane enclosures in host cells. The packaging of the viral genome into the nascent virion is mediated by the nucleocapsid (N) protein, but the underlying mechanism remains unclear. Here, we show that the N protein forms biomolecular condensates with viral genomic RNA both in vitro and in mammalian cells. While the N protein forms spherical assemblies with homopolymeric RNA substrates that do not form base pairing interactions, it forms asymmetric condensates with viral RNA strands. Cross-linking mass spectrometry (CLMS) identified a region that drives interactions between N proteins in condensates, and deletion of this region disrupts phase separation. We also identified small molecules that alter the size and shape of N protein condensates and inhibit the proliferation of SARS-CoV-2 in infected cells. These results suggest that the N protein may utilize biomolecular condensation to package the SARS-CoV-2 RNA genome into a viral particle. Twit Text FOAVip SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA ????PLoS Biol http://www.kidney.de/pget.php?&tw=1&pmid=34634033 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34634033 #FOAvip English Wikipedia <ref>{{Cite pmid|34634033}}</ref> SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic RNA #MMPMID34634033 Jack A; Ferro LS; Trnka MJ; Wehri E; Nadgir A; Nguyenla X; Fox D; Costa K; Stanley S; Schaletzky J; Yildiz A PLoS Biol 2021[Oct]; 19 (10): e3001425 PMID34634033show ga The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection causes Coronavirus Disease 2019 (COVID-19), a pandemic that seriously threatens global health. SARS-CoV-2 propagates by packaging its RNA genome into membrane enclosures in host cells. The packaging of the viral genome into the nascent virion is mediated by the nucleocapsid (N) protein, but the underlying mechanism remains unclear. Here, we show that the N protein forms biomolecular condensates with viral genomic RNA both in vitro and in mammalian cells. While the N protein forms spherical assemblies with homopolymeric RNA substrates that do not form base pairing interactions, it forms asymmetric condensates with viral RNA strands. Cross-linking mass spectrometry (CLMS) identified a region that drives interactions between N proteins in condensates, and deletion of this region disrupts phase separation. We also identified small molecules that alter the size and shape of N protein condensates and inhibit the proliferation of SARS-CoV-2 in infected cells. These results suggest that the N protein may utilize biomolecular condensation to package the SARS-CoV-2 RNA genome into a viral particle. |Animals[MESH] |COVID-19/metabolism/*virology[MESH] |Cell Line, Tumor[MESH] |Chlorocebus aethiops[MESH] |Coronavirus Nucleocapsid Proteins/*metabolism[MESH] |Genome, Viral[MESH] |Genomics[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Nucleocapsid Proteins/genetics[MESH] |Phosphoproteins/metabolism[MESH] |Protein Domains[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/genetics/*metabolism[MESH] |Vero Cells[MESH]SARS-CoV-2 nucleocapsid protein forms condensates with viral genomic R(epmc).pdf DeepDyve Pubget Overpricing