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10.1038/s41467-021-25999-1 http://scihub22266oqcxt.onion/10.1038/s41467-021-25999-1 34584097!8478942!34584097     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34584097&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Commun 2021 ; 12 (1): 5695 Nephropedia Template TP {{tp|p=34584097|t=2021. In vivo structure and dynamics of the SARS-CoV-2 RNA genome |pdf=|usr=}}{{34584097}} gab.com Text In vivo structure and dynamics of the SARS-CoV-2 RNA genome JO: Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34584097 #FOAvip The dynamics of SARS-CoV-2 RNA structure and their functional relevance are largely unknown. Here we develop a simplified SPLASH assay and comprehensively map the in vivo RNA-RNA interactome of SARS-CoV-2 genome across viral life cycle. We report canonical and alternative structures including 5'-UTR and 3'-UTR, frameshifting element (FSE) pseudoknot and genome cyclization in both cells and virions. We provide direct evidence of interactions between Transcription Regulating Sequences, which facilitate discontinuous transcription. In addition, we reveal alternative short and long distance arches around FSE. More importantly, we find that within virions, while SARS-CoV-2 genome RNA undergoes intensive compaction, genome domains remain stable but with strengthened demarcation of local domains and weakened global cyclization. Taken together, our analysis reveals the structural basis for the regulation of replication, discontinuous transcription and translational frameshifting, the alternative conformations and the maintenance of global genome organization during the whole life cycle of SARS-CoV-2, which we anticipate will help develop better antiviral strategies. Twit Text FOAVip In vivo structure and dynamics of the SARS-CoV-2 RNA genome ????Nat Commun http://www.kidney.de/pget.php?&tw=1&pmid=34584097 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34584097 #FOAvip English Wikipedia <ref>{{Cite pmid|34584097}}</ref> In vivo structure and dynamics of the SARS-CoV-2 RNA genome #MMPMID34584097 Zhang Y; Huang K; Xie D; Lau JY; Shen W; Li P; Wang D; Zou Z; Shi S; Ren H; Wang Y; Mao Y; Jin M; Kudla G; Zhao Z Nat Commun 2021[Sep]; 12 (1): 5695 PMID34584097show ga The dynamics of SARS-CoV-2 RNA structure and their functional relevance are largely unknown. Here we develop a simplified SPLASH assay and comprehensively map the in vivo RNA-RNA interactome of SARS-CoV-2 genome across viral life cycle. We report canonical and alternative structures including 5'-UTR and 3'-UTR, frameshifting element (FSE) pseudoknot and genome cyclization in both cells and virions. We provide direct evidence of interactions between Transcription Regulating Sequences, which facilitate discontinuous transcription. In addition, we reveal alternative short and long distance arches around FSE. More importantly, we find that within virions, while SARS-CoV-2 genome RNA undergoes intensive compaction, genome domains remain stable but with strengthened demarcation of local domains and weakened global cyclization. Taken together, our analysis reveals the structural basis for the regulation of replication, discontinuous transcription and translational frameshifting, the alternative conformations and the maintenance of global genome organization during the whole life cycle of SARS-CoV-2, which we anticipate will help develop better antiviral strategies. |Animals[MESH] |COVID-19/virology[MESH] |Chlorocebus aethiops[MESH] |Frameshifting, Ribosomal/*genetics[MESH] |Genome, Viral/*genetics[MESH] |Humans[MESH] |RNA, Viral/*genetics[MESH] |RNA-Seq[MESH] |SARS-CoV-2/*genetics[MESH] |Transcription, Genetic[MESH] |Vero Cells[MESH]In vivo structure and dynamics of the SARS-CoV-2 RNA genome (epmc).pdf DeepDyve Pubget Overpricing