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10.3390/cells10092206 http://scihub22266oqcxt.onion/10.3390/cells10092206 34571855!8465121!34571855     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cells 2021 ; 10 (9): ä Nephropedia Template TP {{tp|p=34571855|t=2021. Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SARS-CoV-2 |pdf=|usr=}}{{34571855}} gab.com Text Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SARS-CoV-2 JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=34571855 #FOAvip The cellular immune response plays an important role in COVID-19, caused by SARS-CoV-2. This feature makes use of in vitro models' useful tools to evaluate vaccines and biopharmaceutical effects. Here, we developed a two-step model to evaluate the cellular immune response after SARS-CoV-2 infection-induced or spike protein stimulation in peripheral blood mononuclear cells (PBMC) from both unexposed and COVID-19 (primo-infected) individuals (Step1). Moreover, the supernatants of these cultures were used to evaluate its effects on lung cell lines (A549) (Step2). When PBMC from the unexposed were infected by SARS-CoV-2, cytotoxic natural killer and nonclassical monocytes expressing inflammatory cytokines genes were raised. The supernatant of these cells can induce apoptosis of A549 cells (mock vs. Step2 [mean]: 6.4% x 17.7%). Meanwhile, PBMCs from primo-infected presented their memory CD4(+) T cells activated with a high production of IFNG and antiviral genes. Supernatant from past COVID-19 subjects contributed to reduce apoptosis (mock vs. Step2 [ratio]: 7.2 x 1.4) and to elevate the antiviral activity (iNOS) of A549 cells (mock vs. Step2 [mean]: 31.5% x 55.7%). Our findings showed features of immune primary cells and lung cell lines response after SARS-CoV-2 or spike protein stimulation that can be used as an in vitro model to study the immunity effects after SARS-CoV-2 antigen exposure. Twit Text FOAVip Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SARS-CoV-2 ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=34571855 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34571855 #FOAvip English Wikipedia <ref>{{Cite pmid|34571855}}</ref> Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SARS-CoV-2 #MMPMID34571855 Melgaco JG; Azamor T; Silva AMV; Linhares JHR; Dos Santos TP; Mendes YS; de Lima SMB; Fernandes CB; da Silva J; de Souza AF; Tubarao LN; Brito E Cunha D; Pereira TBS; Menezes CEL; Miranda MD; Matos AR; Caetano BC; Martins JSCC; Calvo TL; Rodrigues NF; Sacramento CQ; Siqueira MM; Moraes MO; Missailidis S; Neves PCC; Ano Bom APD Cells 2021[Aug]; 10 (9): ä PMID34571855show ga The cellular immune response plays an important role in COVID-19, caused by SARS-CoV-2. This feature makes use of in vitro models' useful tools to evaluate vaccines and biopharmaceutical effects. Here, we developed a two-step model to evaluate the cellular immune response after SARS-CoV-2 infection-induced or spike protein stimulation in peripheral blood mononuclear cells (PBMC) from both unexposed and COVID-19 (primo-infected) individuals (Step1). Moreover, the supernatants of these cultures were used to evaluate its effects on lung cell lines (A549) (Step2). When PBMC from the unexposed were infected by SARS-CoV-2, cytotoxic natural killer and nonclassical monocytes expressing inflammatory cytokines genes were raised. The supernatant of these cells can induce apoptosis of A549 cells (mock vs. Step2 [mean]: 6.4% x 17.7%). Meanwhile, PBMCs from primo-infected presented their memory CD4(+) T cells activated with a high production of IFNG and antiviral genes. Supernatant from past COVID-19 subjects contributed to reduce apoptosis (mock vs. Step2 [ratio]: 7.2 x 1.4) and to elevate the antiviral activity (iNOS) of A549 cells (mock vs. Step2 [mean]: 31.5% x 55.7%). Our findings showed features of immune primary cells and lung cell lines response after SARS-CoV-2 or spike protein stimulation that can be used as an in vitro model to study the immunity effects after SARS-CoV-2 antigen exposure. |*Immunity, Cellular[MESH] |*Models, Biological[MESH] |Adult[MESH] |Alveolar Epithelial Cells/virology[MESH] |COVID-19/blood/genetics/*immunology/*virology[MESH] |Cytokines/genetics/metabolism[MESH] |Female[MESH] |Gene Expression Regulation[MESH] |Humans[MESH] |Immunologic Memory/immunology[MESH] |Killer Cells, Natural/immunology[MESH] |Leukocytes, Mononuclear/virology[MESH] |Male[MESH] |Middle Aged[MESH] |Phenotype[MESH] |SARS-CoV-2/*physiology[MESH] |T-Lymphocytes/immunology[MESH] |Virus Replication/physiology[MESH]Two-Step In Vitro Model to Evaluate the Cellular Immune Response to SA(epmc).pdf DeepDyve Pubget Overpricing