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10.1080/15459624.2021.1985727

http://scihub22266oqcxt.onion/10.1080/15459624.2021.1985727
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suck abstract from ncbi


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pmid34569911      J+Occup+Environ+Hyg 2021 ; 18 (12): 570-578
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  • Persistence of SARS-Co-V-2 on N95 filtering facepiece respirators: implications for reuse #MMPMID34569911
  • Fisher EM; Kuhlman MR; Choi YW; Jordan TL; Sunderman M
  • J Occup Environ Hyg 2021[Dec]; 18 (12): 570-578 PMID34569911show ga
  • In response to the shortage of N95 filtering facepiece respirators for healthcare workers during the COVID-19 pandemic, the Centers for Disease Control and Prevention issued guidance for extended use and limited reuse of N95 FFRs to conserve supply. Previously worn N95 filtering facepiece respirators can serve as a source of pathogens, which can be transferred to the wearer while doffing and donning a respirator when practicing reuse. When practicing limited filtering facepiece respirators reuse, to reduce the risk of self-contamination, the Centers for Disease Control and Prevention recommends storing filtering facepiece respirators for five days between uses to allow for the decay of viable pathogens including SARS-CoV-2. This study assesses the persistence of the SARS-CoV-2 strain USA-WA1/2020 on N95 filtering facepiece respirators under controlled storage conditions for up to 5 days to inform the Centers for Disease Control and Prevention guidance. Coupons excised from six N95 filtering facepiece respirator models and glass slide coverslips were inoculated with the virus in a defined culture medium and in human saliva and stored at 20 degrees C and 20%, 45%, and 75% relative humidity. Statistically significant differences in SARS-CoV-2 half-lives were measured among the tested humidity levels with half-lives decreasing from an average of approximately 30 hr at 20% relative humidity to approximately 2 hr at 75% relative humidity. Significant differences in virus half-lives were also observed between the culture medium and saliva suspension media at 20% and 45% relative humidity with half lives up to 2.9 times greater when the virus was suspended in cell culture medium. The 5-day storage strategy, assessed in this study, resulted in a minimum of 93.4% reduction in viable virus for the most challenging condition (20% relative humidity, cell culture medium) and exceeding 99% reduction in virus at all other conditions.
  • |*COVID-19[MESH]
  • |*Respiratory Protective Devices[MESH]
  • |Equipment Reuse[MESH]
  • |Humans[MESH]
  • |N95 Respirators[MESH]
  • |Pandemics[MESH]
  • |SARS-CoV-2[MESH]


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