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10.1021/acs.nanolett.1c02974 http://scihub22266oqcxt.onion/10.1021/acs.nanolett.1c02974 34542296!ä!34542296 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34542296.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       Nano+Lett 2021 ; 21 (19): 8393-8400 Nephropedia Template TP {{tp|p=34542296|t=2021. Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores |pdf=|usr=}}{{34542296}} gab.com Text Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores JO: Nano Lett http://www.kidney.de/pget.php?&tw=1&pmid=34542296 #FOAvip The outbreak of the SARS-CoV-2 caused the disease COVID-19 to spread globally. Specific and sensitive detection of SARS-CoV-2 facilitates early intervention and prevents the disease from spreading. Here, we present a solid-state CRISPR-Cas12a-assisted nanopore (SCAN) sensing strategy for the specific detection of SARS-CoV-2. We introduced a nanopore-sized counting method to measure the cleavage ratio of reporters, which is used as a criterion for positive/negative classification. A kinetic cleavage model was developed and validated to predict the reporter size distributions. The model revealed the trade-offs between sensitivity, turnaround time, and false-positive rate of the SARS-CoV-2 SCAN. With preamplification and a 30 min CRISPR Cas12a assay, we achieved excellent specificity against other common human coronaviruses and a limit of detection of 13.5 copies/muL (22.5 aM) of viral RNA at a confidence level of 95%. These results suggested that the SCAN could provide a rapid, sensitive, and specific analysis of SARS-CoV-2. Twit Text FOAVip Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores ????Nano Lett http://www.kidney.de/pget.php?&tw=1&pmid=34542296 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34542296 #FOAvip English Wikipedia <ref>{{Cite pmid|34542296}}</ref> Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopores #MMPMID34542296 Nouri R; Jiang Y; Tang Z; Lian XL; Guan W Nano Lett 2021[Oct]; 21 (19): 8393-8400 PMID34542296show ga The outbreak of the SARS-CoV-2 caused the disease COVID-19 to spread globally. Specific and sensitive detection of SARS-CoV-2 facilitates early intervention and prevents the disease from spreading. Here, we present a solid-state CRISPR-Cas12a-assisted nanopore (SCAN) sensing strategy for the specific detection of SARS-CoV-2. We introduced a nanopore-sized counting method to measure the cleavage ratio of reporters, which is used as a criterion for positive/negative classification. A kinetic cleavage model was developed and validated to predict the reporter size distributions. The model revealed the trade-offs between sensitivity, turnaround time, and false-positive rate of the SARS-CoV-2 SCAN. With preamplification and a 30 min CRISPR Cas12a assay, we achieved excellent specificity against other common human coronaviruses and a limit of detection of 13.5 copies/muL (22.5 aM) of viral RNA at a confidence level of 95%. These results suggested that the SCAN could provide a rapid, sensitive, and specific analysis of SARS-CoV-2. |*COVID-19[MESH] |*Nanopores[MESH] |CRISPR-Cas Systems/genetics[MESH] |Humans[MESH] |Nucleic Acid Amplification Techniques[MESH] |SARS-CoV-2[MESH]Detection of SARS-CoV-2 with Solid-State CRISPR-Cas12a-Assisted Nanopo(epmc).pdf DeepDyve Pubget Overpricing