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10.1016/j.xpro.2021.100851

http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100851
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suck abstract from ncbi


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pmid34541555      STAR+Protoc 2021 ; 2 (4): 100851
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  • A new flow cytometry assay to measure antibody-dependent cellular cytotoxicity against SARS-CoV-2 Spike-expressing cells #MMPMID34541555
  • Beaudoin-Bussieres G; Richard J; Prevost J; Goyette G; Finzi A
  • STAR Protoc 2021[Dec]; 2 (4): 100851 PMID34541555show ga
  • Antibodies can engage specific receptors at the surface of effector cells and mediate several functions beyond viral neutralization. Increasing evidence suggests that Fc-mediated effector functions, such as antibody-dependent cellular cytotoxicity (ADCC), have an important role in protection against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. We engineered a cell line stably expressing a GFP-tagged SARS-CoV-2 spike to measure ADCC. This protocol provides an optimized way of measuring ADCC activity mediated by anti-SARS-CoV-2 Spike monoclonal antibodies or plasma from previously infected or vaccinated individuals. For complete details on the use and execution of this protocol, please refer to Anand et al. (2021b).
  • |Antibodies, Monoclonal/*immunology[MESH]
  • |Antibodies, Neutralizing/*blood/immunology[MESH]
  • |Antibodies, Viral/*blood/immunology[MESH]
  • |Antibody-Dependent Cell Cytotoxicity/*immunology[MESH]
  • |COVID-19/*immunology/prevention & control/virology[MESH]
  • |Flow Cytometry/*methods[MESH]
  • |Humans[MESH]


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