Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1002/jmv.27336 http://scihub22266oqcxt.onion/10.1002/jmv.27336 34524690!8662006!34524690     free     free     free Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 217.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       J+Med+Virol 2022 ; 94 (1): 327-334 Nephropedia Template TP {{tp|p=34524690|t=2022. Assessment of two-pool multiplex long-amplicon nanopore sequencing of SARS-CoV-2 |pdf=|usr=}}{{34524690}} gab.com Text Assessment of two-pool multiplex long-amplicon nanopore sequencing of SARS-CoV-2 JO: J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34524690 #FOAvip Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays an important role in COVID-19 pandemic control and elimination efforts, especially by elucidating its global transmission network and illustrating its viral evolution. The deployment of multiplex PCR assays that target SARS-CoV-2 followed by either massively parallel or nanopore sequencing is a widely-used strategy to obtain genome sequences from primary samples. However, multiplex PCR-based sequencing carries an inherent bias of sequencing depth among different amplicons, which may cause uneven coverage. Here we developed a two-pool, long-amplicon 36-plex PCR primer panel with ~1000-bp amplicon lengths for full-genome sequencing of SARS-CoV-2. We validated the panel by assessing nasopharyngeal swab samples with a <30 quantitative reverse transcription PCR cycle threshold value and found that >/=90% of viral genomes could be covered with high sequencing depths (>/=20% mean depth). In comparison, the widely-used ARTIC panel yielded 79%-88% high-depth genome regions. We estimated that ~5 Mbp nanopore sequencing data may ensure a >95% viral genome coverage with a >/=10-fold depth and may generate reliable genomes at consensus sequence levels. Nanopore sequencing yielded false-positive variations with frequencies of supporting reads <0.8, and the sequencing errors mostly occurred on the 5' or 3' ends of reads. Thus, nanopore sequencing could not elucidate intra-host viral diversity. Twit Text FOAVip Assessment of two-pool multiplex long-amplicon nanopore sequencing of SARS-CoV-2 ????J Med Virol http://www.kidney.de/pget.php?&tw=1&pmid=34524690 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34524690 #FOAvip English Wikipedia <ref>{{Cite pmid|34524690}}</ref> Assessment of two-pool multiplex long-amplicon nanopore sequencing of SARS-CoV-2 #MMPMID34524690 Liu H; Li J; Lin Y; Bo X; Song H; Li K; Li P; Ni M J Med Virol 2022[Jan]; 94 (1): 327-334 PMID34524690show ga Genomic surveillance of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) plays an important role in COVID-19 pandemic control and elimination efforts, especially by elucidating its global transmission network and illustrating its viral evolution. The deployment of multiplex PCR assays that target SARS-CoV-2 followed by either massively parallel or nanopore sequencing is a widely-used strategy to obtain genome sequences from primary samples. However, multiplex PCR-based sequencing carries an inherent bias of sequencing depth among different amplicons, which may cause uneven coverage. Here we developed a two-pool, long-amplicon 36-plex PCR primer panel with ~1000-bp amplicon lengths for full-genome sequencing of SARS-CoV-2. We validated the panel by assessing nasopharyngeal swab samples with a <30 quantitative reverse transcription PCR cycle threshold value and found that >/=90% of viral genomes could be covered with high sequencing depths (>/=20% mean depth). In comparison, the widely-used ARTIC panel yielded 79%-88% high-depth genome regions. We estimated that ~5 Mbp nanopore sequencing data may ensure a >95% viral genome coverage with a >/=10-fold depth and may generate reliable genomes at consensus sequence levels. Nanopore sequencing yielded false-positive variations with frequencies of supporting reads <0.8, and the sequencing errors mostly occurred on the 5' or 3' ends of reads. Thus, nanopore sequencing could not elucidate intra-host viral diversity. |COVID-19[MESH] |Genome, Viral/*genetics[MESH] |High-Throughput Nucleotide Sequencing/methods[MESH] |Humans[MESH] |Multiplex Polymerase Chain Reaction/*methods[MESH] |Nanopore Sequencing/*methods[MESH] |Nasopharynx/virology[MESH] |RNA, Viral/genetics[MESH] |SARS-CoV-2/*genetics[MESH] |Sequence Analysis, RNA/methods[MESH]Assessment of two-pool multiplex long-amplicon nanopore sequencing of (epmc).pdf DeepDyve Pubget Overpricing