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10.3390/v13081506

http://scihub22266oqcxt.onion/10.3390/v13081506
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34452371!8402769!34452371
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suck abstract from ncbi


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pmid34452371      Viruses 2021 ; 13 (8): ä
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  • Intravenous, Intratracheal, and Intranasal Inoculation of Swine with SARS-CoV-2 #MMPMID34452371
  • Buckley A; Falkenberg S; Martins M; Laverack M; Palmer MV; Lager K; Diel DG
  • Viruses 2021[Jul]; 13 (8): ä PMID34452371show ga
  • Since the emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the susceptibility of animals and their potential to act as reservoirs or intermediate hosts for the virus has been of significant interest. Pigs are susceptible to multiple coronaviruses and have been used as an animal model for other human infectious diseases. Research groups have experimentally challenged swine with human SARS-CoV-2 isolates with results suggesting limited to no viral replication. For this study, a SARS-CoV-2 isolate obtained from a tiger which is identical to human SARS-CoV-2 isolates detected in New York City and contains the D614G S mutation was utilized for inoculation. Pigs were challenged via intravenous, intratracheal, or intranasal routes of inoculation (n = 4/route). No pigs developed clinical signs, but at least one pig in each group had one or more PCR positive nasal/oral swabs or rectal swabs after inoculation. All pigs in the intravenous group developed a transient neutralizing antibody titer, but only three other challenged pigs developed titers greater than 1:8. No gross or histologic changes were observed in tissue samples collected at necropsy. In addition, no PCR positive samples were positive by virus isolation. Inoculated animals were unable to transmit virus to naive contact animals. The data from this experiment as well as from other laboratories supports that swine are not likely to play a role in the epidemiology and spread of SARS-CoV-2.
  • |Administration, Intranasal[MESH]
  • |Administration, Intravenous[MESH]
  • |Animals[MESH]
  • |Antibodies, Neutralizing/blood[MESH]
  • |Antibodies, Viral/blood[MESH]
  • |COVID-19/blood/immunology/*virology[MESH]
  • |Disease Models, Animal[MESH]
  • |Humans[MESH]
  • |Mouth/virology[MESH]
  • |Nose/virology[MESH]
  • |SARS-CoV-2/genetics/*physiology[MESH]
  • |Swine[MESH]
  • |Trachea/virology[MESH]


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