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10.3390/ijms22168744 http://scihub22266oqcxt.onion/10.3390/ijms22168744 34445449!8395930!34445449     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34445449&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215       Int+J+Mol+Sci 2021 ; 22 (16): ? Nephropedia Template TP {{tp|p=34445449|t=2021. Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current |pdf=|usr=}}{{34445449}} gab.com Text Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current JO: Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34445449 #FOAvip The cardiac Mg(2+)-sensitive, TRPM6, and TRPM7-like channels remain undefined, especially with the uncertainty regarding TRPM6 expression in cardiomyocytes. Additionally, their contribution to the cardiac action potential (AP) profile is unclear. Immunofluorescence assays showed the expression of the TRPM6 and TRPM7 proteins in isolated pig atrial and ventricular cardiomyocytes, of which the expression was modulated by incubation in extracellular divalent cation-free conditions. In patch clamp studies of cells dialyzed with solutions containing zero intracellular Mg(2+) concentration ([Mg(2+)](i)) to activate the Mg(2+)-sensitive channels, raising extracellular [Mg(2+)] ([Mg(2+)](o)) from the 0.9-mM baseline to 7.2 mM prolonged the AP duration (APD). In contrast, no such effect was observed in cells dialyzed with physiological [Mg(2+)](i). Under voltage clamp, in cells dialyzed with zero [Mg(2+)](i), depolarizing ramps induced an outward-rectifying current, which was suppressed by raising [Mg(2+)](o) and was absent in cells dialyzed with physiological [Mg(2+)](i). In cells dialyzed with physiological [Mg(2+)](i), raising [Mg(2+)](o) decreased the L-type Ca(2+) current and the total delayed-rectifier current but had no effect on the APD. These results suggest a co-expression of the TRPM6 and TRPM7 proteins in cardiomyocytes, which are therefore the molecular candidates for the native cardiac Mg(2+)-sensitive channels, and also suggest that the cardiac Mg(2+)-sensitive current shortens the APD, with potential implications in arrhythmogenesis. Twit Text FOAVip Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current ????Int J Mol Sci http://www.kidney.de/pget.php?&tw=1&pmid=34445449 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34445449 #FOAvip English Wikipedia <ref>{{Cite pmid|34445449}}</ref> Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Sensitive TRPM6 and TRPM7-like Current #MMPMID34445449 Gwanyanya A; Andriule I; Istrate BM; Easmin F; Mubagwa K; Macianskiene R Int J Mol Sci 2021[Aug]; 22 (16): ? PMID34445449show ga The cardiac Mg(2+)-sensitive, TRPM6, and TRPM7-like channels remain undefined, especially with the uncertainty regarding TRPM6 expression in cardiomyocytes. Additionally, their contribution to the cardiac action potential (AP) profile is unclear. Immunofluorescence assays showed the expression of the TRPM6 and TRPM7 proteins in isolated pig atrial and ventricular cardiomyocytes, of which the expression was modulated by incubation in extracellular divalent cation-free conditions. In patch clamp studies of cells dialyzed with solutions containing zero intracellular Mg(2+) concentration ([Mg(2+)](i)) to activate the Mg(2+)-sensitive channels, raising extracellular [Mg(2+)] ([Mg(2+)](o)) from the 0.9-mM baseline to 7.2 mM prolonged the AP duration (APD). In contrast, no such effect was observed in cells dialyzed with physiological [Mg(2+)](i). Under voltage clamp, in cells dialyzed with zero [Mg(2+)](i), depolarizing ramps induced an outward-rectifying current, which was suppressed by raising [Mg(2+)](o) and was absent in cells dialyzed with physiological [Mg(2+)](i). In cells dialyzed with physiological [Mg(2+)](i), raising [Mg(2+)](o) decreased the L-type Ca(2+) current and the total delayed-rectifier current but had no effect on the APD. These results suggest a co-expression of the TRPM6 and TRPM7 proteins in cardiomyocytes, which are therefore the molecular candidates for the native cardiac Mg(2+)-sensitive channels, and also suggest that the cardiac Mg(2+)-sensitive current shortens the APD, with potential implications in arrhythmogenesis. |*Action Potentials[MESH] |Animals[MESH] |Cations, Divalent[MESH] |Magnesium/*metabolism[MESH] |Myocytes, Cardiac/*metabolism/physiology[MESH] |Sus scrofa/metabolism/physiology[MESH]Modulation of the Cardiac Myocyte Action Potential by the Magnesium-Se(epmc).pdf DeepDyve Pubget Overpricing