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10.3390/cells10081900 http://scihub22266oqcxt.onion/10.3390/cells10081900 34440669!8394956!34440669     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34440669&cmd=llinks): Failed to open stream: HTTP request failed! HTTP/1.1 429 Too Many Requests in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 215 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Cells 2021 ; 10 (8): ä Nephropedia Template TP {{tp|p=34440669|t=2021. Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 Patients |pdf=|usr=}}{{34440669}} gab.com Text Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 Patients JO: Cells http://www.kidney.de/pget.php?&tw=1&pmid=34440669 #FOAvip Multiorgan tropism of SARS-CoV-2 has previously been shown for several major organs. We have comprehensively analyzed 25 different formalin-fixed paraffin-embedded (FFPE) tissues/organs from autopsies of fatal COVID-19 cases (n = 8), using histopathological assessment, detection of SARS-CoV-2 RNA using polymerase chain reaction and RNA in situ hybridization, viral protein using immunohistochemistry, and virus particles using transmission electron microscopy. SARS-CoV-2 RNA was mainly localized in epithelial cells across all organs. Next to lung, trachea, kidney, heart, or liver, viral RNA was also found in tonsils, salivary glands, oropharynx, thyroid, adrenal gland, testicles, prostate, ovaries, small bowel, lymph nodes, skin and skeletal muscle. Viral RNA was predominantly found in cells expressing ACE2, TMPRSS2, or both. The SARS-CoV-2 replicating RNA was also detected in these organs. Immunohistochemistry and electron microscopy were not suitable for reliable and specific SARS-CoV-2 detection in autopsies. These findings were validated using in situ hybridization on external COVID-19 autopsy samples (n = 9). Apart from the lung, correlation of viral detection and histopathological assessment did not reveal any specific alterations that could be attributed to SARS-CoV-2. In summary, SARS-CoV-2 and its replication could be observed across all organ systems, which co-localizes with ACE2 and TMPRSS2 mainly in epithelial but also in mesenchymal and endothelial cells. Apart from the respiratory tract, no specific (histo-)morphologic alterations could be assigned to the SARS-CoV-2 infection. Twit Text FOAVip Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 Patients ????Cells http://www.kidney.de/pget.php?&tw=1&pmid=34440669 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34440669 #FOAvip English Wikipedia <ref>{{Cite pmid|34440669}}</ref> Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 Patients #MMPMID34440669 Wong DWL; Klinkhammer BM; Djudjaj S; Villwock S; Timm MC; Buhl EM; Wucherpfennig S; Cacchi C; Braunschweig T; Knuchel-Clarke R; Jonigk D; Werlein C; Bulow RD; Dahl E; von Stillfried S; Boor P Cells 2021[Jul]; 10 (8): ä PMID34440669show ga Multiorgan tropism of SARS-CoV-2 has previously been shown for several major organs. We have comprehensively analyzed 25 different formalin-fixed paraffin-embedded (FFPE) tissues/organs from autopsies of fatal COVID-19 cases (n = 8), using histopathological assessment, detection of SARS-CoV-2 RNA using polymerase chain reaction and RNA in situ hybridization, viral protein using immunohistochemistry, and virus particles using transmission electron microscopy. SARS-CoV-2 RNA was mainly localized in epithelial cells across all organs. Next to lung, trachea, kidney, heart, or liver, viral RNA was also found in tonsils, salivary glands, oropharynx, thyroid, adrenal gland, testicles, prostate, ovaries, small bowel, lymph nodes, skin and skeletal muscle. Viral RNA was predominantly found in cells expressing ACE2, TMPRSS2, or both. The SARS-CoV-2 replicating RNA was also detected in these organs. Immunohistochemistry and electron microscopy were not suitable for reliable and specific SARS-CoV-2 detection in autopsies. These findings were validated using in situ hybridization on external COVID-19 autopsy samples (n = 9). Apart from the lung, correlation of viral detection and histopathological assessment did not reveal any specific alterations that could be attributed to SARS-CoV-2. In summary, SARS-CoV-2 and its replication could be observed across all organ systems, which co-localizes with ACE2 and TMPRSS2 mainly in epithelial but also in mesenchymal and endothelial cells. Apart from the respiratory tract, no specific (histo-)morphologic alterations could be assigned to the SARS-CoV-2 infection. |Aged[MESH] |Angiotensin-Converting Enzyme 2/*genetics[MESH] |Autopsy[MESH] |COVID-19/genetics/*metabolism/pathology/virology[MESH] |Endothelial Cells/*metabolism/pathology/virology[MESH] |Female[MESH] |Gene Expression Regulation[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Organ Specificity[MESH] |RNA, Viral/*analysis[MESH] |SARS-CoV-2/*physiology[MESH] |Serine Endopeptidases/*genetics[MESH]Multisystemic Cellular Tropism of SARS-CoV-2 in Autopsies of COVID-19 (epmc).pdf DeepDyve Pubget Overpricing