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10.1080/21655979.2021.1955509 http://scihub22266oqcxt.onion/10.1080/21655979.2021.1955509 34436976!8806791!34436976     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34436976&cmd=llinks): Failed to open stream: HTTP request failed! 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An easy method for developing fusion enabled SARS-CoV2 virus fusion mimic (SCFM), bypassing the need of Bio Safety Level (BSL) facility |pdf=|usr=}}{{34436976}} gab.com Text An easy method for developing fusion enabled SARS-CoV2 virus fusion mimic (SCFM), bypassing the need of Bio Safety Level (BSL) facility JO: Bioengineered http://www.kidney.de/pget.php?&tw=1&pmid=34436976 #FOAvip Widespread infection due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has led to a global pandemic. Currently, various approaches are being taken up to develop vaccines and therapeutics to treat SARS-CoV2 infection. Consequently, the S protein has become an important target protein for developing vaccines and therapeutics against SARS-CoV2. However, the highly infective nature of SARS-CoV2 restricts experimentation with the virus to highly secure BSL3 facilities. The availability of fusion-enabled, nonreplicating, and nonbiohazardous mimics of SARS-CoV2 virus fusion, containing the viral S or S and M protein in their native conformation on mammalian cells, can serve as a useful substitute for studying viral fusion for testing various inhibitors of viral fusion. This would avoid the use of the BSL3 facility for fusion studies required to develop therapeutics. In the present study, we have developed SARS-CoV2 virus fusion mimics (SCFMs) using mammalian cells transfected with constructs coding for S or S and M protein. The fusogenic property of the mimic(s) and their interaction with the functional human ACE2 receptors was confirmed experimentally. We have also shown that such mimics can easily be used in an inhibition assay. These mimic(s) can be easily prepared on a large scale, and such SCFMs can serve as an invaluable resource for viral fusion inhibition assays and in vitro screening of antiviral agents, which can be shared/handled between labs/facilities without worrying about any biohazard while working under routine laboratory conditions, avoiding the use of BSL3 laboratory.Abbreviations :SCFM: SARS-CoV2 Virus Fusion Mimic; ACE2: Angiotensin-Converting Enzyme 2; hACE2: Human Angiotensin-Converting enzyme 2; MEF: Mouse Embryonic Fibroblasts; HBSS: Hanks Balanced Salt Solution; FBS: Fetal Bovine Serum. Twit Text FOAVip An easy method for developing fusion enabled SARS-CoV2 virus fusion mimic (SCFM), bypassing the need of Bio Safety Level (BSL) facility ????Bioengineered http://www.kidney.de/pget.php?&tw=1&pmid=34436976 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34436976 #FOAvip English Wikipedia <ref>{{Cite pmid|34436976}}</ref> An easy method for developing fusion enabled SARS-CoV2 virus fusion mimic (SCFM), bypassing the need of Bio Safety Level (BSL) facility #MMPMID34436976 Das A; Dutta S; Sharma D; Pal A; Ganguli N; Majumdar SS Bioengineered 2021[Dec]; 12 (1): 4407-4419 PMID34436976show ga Widespread infection due to severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) has led to a global pandemic. Currently, various approaches are being taken up to develop vaccines and therapeutics to treat SARS-CoV2 infection. Consequently, the S protein has become an important target protein for developing vaccines and therapeutics against SARS-CoV2. However, the highly infective nature of SARS-CoV2 restricts experimentation with the virus to highly secure BSL3 facilities. The availability of fusion-enabled, nonreplicating, and nonbiohazardous mimics of SARS-CoV2 virus fusion, containing the viral S or S and M protein in their native conformation on mammalian cells, can serve as a useful substitute for studying viral fusion for testing various inhibitors of viral fusion. This would avoid the use of the BSL3 facility for fusion studies required to develop therapeutics. In the present study, we have developed SARS-CoV2 virus fusion mimics (SCFMs) using mammalian cells transfected with constructs coding for S or S and M protein. The fusogenic property of the mimic(s) and their interaction with the functional human ACE2 receptors was confirmed experimentally. We have also shown that such mimics can easily be used in an inhibition assay. These mimic(s) can be easily prepared on a large scale, and such SCFMs can serve as an invaluable resource for viral fusion inhibition assays and in vitro screening of antiviral agents, which can be shared/handled between labs/facilities without worrying about any biohazard while working under routine laboratory conditions, avoiding the use of BSL3 laboratory.Abbreviations :SCFM: SARS-CoV2 Virus Fusion Mimic; ACE2: Angiotensin-Converting Enzyme 2; hACE2: Human Angiotensin-Converting enzyme 2; MEF: Mouse Embryonic Fibroblasts; HBSS: Hanks Balanced Salt Solution; FBS: Fetal Bovine Serum. |Angiotensin-Converting Enzyme 2/genetics/metabolism[MESH] |Animals[MESH] |Antibodies, Neutralizing/*pharmacology[MESH] |Chlorocebus aethiops[MESH] |Containment of Biohazards/*methods[MESH] |Embryo, Mammalian[MESH] |Fibroblasts/drug effects/virology[MESH] |Gene Expression[MESH] |Genes, Reporter[MESH] |Green Fluorescent Proteins/genetics/metabolism[MESH] |HEK293 Cells[MESH] |Humans[MESH] |Luminescent Proteins/genetics/metabolism[MESH] |MCF-7 Cells[MESH] |Mice[MESH] |Molecular Mimicry[MESH] |Plasmids/chemistry/metabolism[MESH] |Primary Cell Culture[MESH] |Protein Binding[MESH] |Receptors, Virus/genetics/metabolism[MESH] |SARS-CoV-2/drug effects/*genetics/metabolism[MESH] |Spike Glycoprotein, Coronavirus/*antagonists & inhibitors/genetics/metabolism[MESH] |Transfection[MESH] |Vero Cells[MESH] |Viral Matrix Proteins/*antagonists & inhibitors/genetics/metabolism[MESH]An easy method for developing fusion enabled SARS-CoV2 virus fusion mi(epmc).pdf DeepDyve Pubget Overpricing