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10.3390/bios11080251 http://scihub22266oqcxt.onion/10.3390/bios11080251 34436052!8392451!34436052     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34436052&cmd=llinks): Failed to open stream: HTTP request failed! 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SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine |pdf=|usr=}}{{34436052}} gab.com Text SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine JO: Biosensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34436052 #FOAvip Cell-based assays are a valuable tool for examination of virus-host cell interactions and drug discovery processes, allowing for a more physiological setting compared to biochemical assays. Despite the fact that cell-based SPR assays are label-free and thus provide all the associated benefits, they have never been used to study viral growth kinetics and to predict drug antiviral response in cells. In this study, we prove the concept that the cell-based SPR assay can be applied in the kinetic analysis of the early stages of viral infection of cells and the antiviral drug activity in the infected cells. For this purpose, cells immobilized on the SPR slides were infected with human coronavirus HCov-229E and treated with hydroxychloroquine. The SPR response was measured at different time intervals within the early stages of infection. Methyl Thiazolyl Tetrazolium (MTT) assay was used to provide the reference data. We found that the results of the SPR and MTT assays were consistent, and SPR is a reliable tool in investigating virus-host cell interaction and the mechanism of action of viral inhibitors. SPR assay was more sensitive and accurate in the first hours of infection within the first replication cycle, whereas the MTT assay was not so effective. After the second replication cycle, noise was generated by the destruction of the cell layer and by the remnants of dead cells, and masks useful SPR signals. Twit Text FOAVip SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine ????Biosensors (Basel) http://www.kidney.de/pget.php?&tw=1&pmid=34436052 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34436052 #FOAvip English Wikipedia <ref>{{Cite pmid|34436052}}</ref> SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells Infected with Human Coronavirus and Treated with Hydroxychloroquine #MMPMID34436052 Genova-Kalou P; Dyankov G; Marinov R; Mankov V; Belina E; Kisov H; Strijkova-Kenderova V; Kantardjiev T Biosensors (Basel) 2021[Jul]; 11 (8): ä PMID34436052show ga Cell-based assays are a valuable tool for examination of virus-host cell interactions and drug discovery processes, allowing for a more physiological setting compared to biochemical assays. Despite the fact that cell-based SPR assays are label-free and thus provide all the associated benefits, they have never been used to study viral growth kinetics and to predict drug antiviral response in cells. In this study, we prove the concept that the cell-based SPR assay can be applied in the kinetic analysis of the early stages of viral infection of cells and the antiviral drug activity in the infected cells. For this purpose, cells immobilized on the SPR slides were infected with human coronavirus HCov-229E and treated with hydroxychloroquine. The SPR response was measured at different time intervals within the early stages of infection. Methyl Thiazolyl Tetrazolium (MTT) assay was used to provide the reference data. We found that the results of the SPR and MTT assays were consistent, and SPR is a reliable tool in investigating virus-host cell interaction and the mechanism of action of viral inhibitors. SPR assay was more sensitive and accurate in the first hours of infection within the first replication cycle, whereas the MTT assay was not so effective. After the second replication cycle, noise was generated by the destruction of the cell layer and by the remnants of dead cells, and masks useful SPR signals. |Animals[MESH] |Antiviral Agents/pharmacology/*therapeutic use[MESH] |Chlorocebus aethiops[MESH] |Coronavirus 229E, Human/drug effects/isolation & purification/*physiology[MESH] |Coronavirus Infections/*drug therapy/pathology/virology[MESH] |Humans[MESH] |Hydroxychloroquine/pharmacology/*therapeutic use[MESH] |Kinetics[MESH] |Severity of Illness Index[MESH] |Surface Plasmon Resonance/*methods[MESH]SPR-Based Kinetic Analysis of the Early Stages of Infection in Cells I(epmc).pdf DeepDyve Pubget Overpricing