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10.1371/journal.pone.0244468 http://scihub22266oqcxt.onion/10.1371/journal.pone.0244468 34432798!8386831!34432798     free     free     free Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 231.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 265.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Warning: imagejpeg(C:\Inetpub\vhosts\kidney.de\httpdocs\phplern\34432798.jpg): Failed to open stream: No such file or directory in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 117       PLoS+One 2021 ; 16 (8): e0244468 Nephropedia Template TP {{tp|p=34432798|t=2021. Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals |pdf=|usr=}}{{34432798}} gab.com Text Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals JO: PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34432798 #FOAvip The newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open reading frames [ORFs]) sequences from 45 SARS-CoV-2 clinical samples with varying viral titers. For samples with higher viral loads (cycle threshold value under 33, based on the CDC qPCR assay) complete genomes were generated. Analysis of junction reads revealed regions of differential transcriptional activity among samples. Mixed allelic frequencies along the 20kb ORF1ab gene in one sample, suggested the presence of a defective viral RNA species subpopulation maintained in mixture with functional RNA in one sample. The associated workflow is straightforward, and hybridization-based capture offers an effective and scalable approach for sequencing SARS-CoV-2 from patient samples. Twit Text FOAVip Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals ????PLoS One http://www.kidney.de/pget.php?&tw=1&pmid=34432798 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34432798 #FOAvip English Wikipedia <ref>{{Cite pmid|34432798}}</ref> Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgenomic fragments from COVID-19 individuals #MMPMID34432798 Doddapaneni H; Cregeen SJ; Sucgang R; Meng Q; Qin X; Avadhanula V; Chao H; Menon V; Nicholson E; Henke D; Piedra FA; Rajan A; Momin Z; Kottapalli K; Hoffman KL; Sedlazeck FJ; Metcalf G; Piedra PA; Muzny DM; Petrosino JF; Gibbs RA PLoS One 2021[]; 16 (8): e0244468 PMID34432798show ga The newly emerged and rapidly spreading SARS-CoV-2 causes coronavirus disease 2019 (COVID-19). To facilitate a deeper understanding of the viral biology we developed a capture sequencing methodology to generate SARS-CoV-2 genomic and transcriptome sequences from infected patients. We utilized an oligonucleotide probe-set representing the full-length genome to obtain both genomic and transcriptome (subgenomic open reading frames [ORFs]) sequences from 45 SARS-CoV-2 clinical samples with varying viral titers. For samples with higher viral loads (cycle threshold value under 33, based on the CDC qPCR assay) complete genomes were generated. Analysis of junction reads revealed regions of differential transcriptional activity among samples. Mixed allelic frequencies along the 20kb ORF1ab gene in one sample, suggested the presence of a defective viral RNA species subpopulation maintained in mixture with functional RNA in one sample. The associated workflow is straightforward, and hybridization-based capture offers an effective and scalable approach for sequencing SARS-CoV-2 from patient samples. |COVID-19/*pathology/virology[MESH] |DNA, Complementary/chemistry/metabolism[MESH] |Gene Frequency[MESH] |Genetic Variation[MESH] |Genome, Viral[MESH] |Humans[MESH] |Open Reading Frames/genetics[MESH] |RNA, Viral/genetics/metabolism[MESH] |Real-Time Polymerase Chain Reaction[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Sequence Analysis, DNA/*methods[MESH]Oligonucleotide capture sequencing of the SARS-CoV-2 genome and subgen(epmc).pdf DeepDyve Pubget Overpricing