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10.1021/acsami.1c09309

http://scihub22266oqcxt.onion/10.1021/acsami.1c09309
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34428374!ä!34428374

suck abstract from ncbi


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pmid34428374      ACS+Appl+Mater+Interfaces 2021 ; 13 (35): 41445-41453
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  • Wearable Collector for Noninvasive Sampling of SARS-CoV-2 from Exhaled Breath for Rapid Detection #MMPMID34428374
  • Soto F; Ozen MO; Guimaraes CF; Wang J; Hokanson K; Ahmed R; Reis RL; Paulmurugan R; Demirci U
  • ACS Appl Mater Interfaces 2021[Sep]; 13 (35): 41445-41453 PMID34428374show ga
  • Airborne transmission of exhaled virus can rapidly spread, thereby increasing disease progression from local incidents to pandemics. Due to the COVID-19 pandemic, states and local governments have enforced the use of protective masks in public and work areas to minimize the disease spread. Here, we have leveraged the function of protective face coverings toward COVID-19 diagnosis. We developed a user-friendly, affordable, and wearable collector. This noninvasive platform is integrated into protective masks toward collecting airborne virus in the exhaled breath over the wearing period. A viral sample was sprayed into the collector to model airborne dispersion, and then the enriched pathogen was extracted from the collector for further analytical evaluation. To validate this design, qualitative colorimetric loop-mediated isothermal amplification, quantitative reverse transcription polymerase chain reaction, and antibody-based dot blot assays were performed to detect the presence of SARS-CoV-2. We envision that this platform will facilitate sampling of current SARS-CoV-2 and is potentially broadly applicable to other airborne diseases for future emerging pandemics.
  • |*Masks[MESH]
  • |Air Microbiology[MESH]
  • |Antibodies, Viral/immunology[MESH]
  • |Breath Tests/*instrumentation/methods[MESH]
  • |COVID-19 Testing/*instrumentation/methods[MESH]
  • |Collodion/chemistry[MESH]
  • |Colorimetry/methods[MESH]
  • |Molecular Diagnostic Techniques/methods[MESH]
  • |Nucleic Acid Amplification Techniques/methods[MESH]
  • |Polycarboxylate Cement/chemistry[MESH]
  • |Porosity[MESH]
  • |Proof of Concept Study[MESH]
  • |RNA, Viral/analysis[MESH]
  • |Real-Time Polymerase Chain Reaction/methods[MESH]
  • |SARS-CoV-2/chemistry/*isolation & purification[MESH]


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