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10.1038/s41594-021-00653-y http://scihub22266oqcxt.onion/10.1038/s41594-021-00653-y 34426697!8848339!34426697     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 263.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Nat+Struct+Mol+Biol 2021 ; 28 (9): 747-754 Nephropedia Template TP {{tp|p=34426697|t=2021. Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome |pdf=|usr=}}{{34426697}} gab.com Text Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome JO: Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34426697 #FOAvip Drug discovery campaigns against COVID-19 are beginning to target the SARS-CoV-2 RNA genome. The highly conserved frameshift stimulation element (FSE), required for balanced expression of viral proteins, is a particularly attractive SARS-CoV-2 RNA target. Here we present a 6.9 A resolution cryo-EM structure of the FSE (88 nucleotides, ~28 kDa), validated through an RNA nanostructure tagging method. The tertiary structure presents a topologically complex fold in which the 5' end is threaded through a ring formed inside a three-stem pseudoknot. Guided by this structure, we develop antisense oligonucleotides that impair FSE function in frameshifting assays and knock down SARS-CoV-2 virus replication in A549-ACE2 cells at 100 nM concentration. Twit Text FOAVip Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome ????Nat Struct Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34426697 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34426697 #FOAvip English Wikipedia <ref>{{Cite pmid|34426697}}</ref> Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation element from the SARS-CoV-2 RNA genome #MMPMID34426697 Zhang K; Zheludev IN; Hagey RJ; Haslecker R; Hou YJ; Kretsch R; Pintilie GD; Rangan R; Kladwang W; Li S; Wu MT; Pham EA; Bernardin-Souibgui C; Baric RS; Sheahan TP; D'Souza V; Glenn JS; Chiu W; Das R Nat Struct Mol Biol 2021[Sep]; 28 (9): 747-754 PMID34426697show ga Drug discovery campaigns against COVID-19 are beginning to target the SARS-CoV-2 RNA genome. The highly conserved frameshift stimulation element (FSE), required for balanced expression of viral proteins, is a particularly attractive SARS-CoV-2 RNA target. Here we present a 6.9 A resolution cryo-EM structure of the FSE (88 nucleotides, ~28 kDa), validated through an RNA nanostructure tagging method. The tertiary structure presents a topologically complex fold in which the 5' end is threaded through a ring formed inside a three-stem pseudoknot. Guided by this structure, we develop antisense oligonucleotides that impair FSE function in frameshifting assays and knock down SARS-CoV-2 virus replication in A549-ACE2 cells at 100 nM concentration. |A549 Cells[MESH] |Animals[MESH] |Base Sequence[MESH] |COVID-19/*prevention & control/virology[MESH] |Cell Line, Tumor[MESH] |Chlorocebus aethiops[MESH] |Cryoelectron Microscopy/*methods[MESH] |Frameshift Mutation/*genetics[MESH] |Genome, Viral/genetics[MESH] |Humans[MESH] |Models, Molecular[MESH] |Nucleic Acid Conformation[MESH] |Oligonucleotides, Antisense/*genetics/pharmacology[MESH] |RNA, Viral/chemistry/*genetics/ultrastructure[MESH] |Response Elements/*genetics[MESH] |SARS-CoV-2/*genetics/physiology/ultrastructure[MESH] |Vero Cells[MESH]Cryo-EM and antisense targeting of the 28-kDa frameshift stimulation e(epmc).pdf DeepDyve Pubget Overpricing