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10.1016/j.cca.2021.08.024 http://scihub22266oqcxt.onion/10.1016/j.cca.2021.08.024 34425105!8378065!34425105     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Clin+Chim+Acta 2021 ; 522 (ä): 144-151 Nephropedia Template TP {{tp|p=34425105|t=2021. Harmonization of six quantitative SARS-CoV-2 serological assays using sera of vaccinated subjects |pdf=|usr=}}{{34425105}} gab.com Text Harmonization of six quantitative SARS-CoV-2 serological assays using sera of vaccinated subjects JO: Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=34425105 #FOAvip BACKGROUND AND AIMS: Vaccines, to limit SARS-CoV-2 infection, were produced and reliable assays are needed for their evaluation. The WHO produced an International-Standard (WHO-IS) to facilitate the standardization/comparison of serological methods. The WHO-IS, produced in limited amount, was never tested for reproducibility. This study aims at developing a reproducible and accessible working standard (WS) to complement the WHO-IS. MATERIALS AND METHODS: Sera from vaccinated individuals were used to produce the WSs. The WHO-IS, the WSs and single serum samples (n = 48) were tested on 6 quantitative serological devices. Neutralization assays were performed for the 48 samples and compared with their antibody titers. RESULTS: The WS carry an antibody titer 20-fold higher than the WHO-IS. It was reproducible, showed both good linearity and insignificant intra- and inter-laboratory variability. However, the WSs behave differently from the WHO-IS. Analysis of the 48 samples showed that single correlation factors are not sufficient to harmonize results from different assays. Yet, all the devices predict neutralization activity based on the antibody titer. CONCLUSIONS: A reproducible and highly concentrated WS, specific for IgG against SARS-CoV-2 Spike-glycoprotein was produced. Such characteristics make it particularly suited for the harmonization of commercially available assays and the consequent evaluation of post-vaccinated individuals. Twit Text FOAVip Harmonization of six quantitative SARS-CoV-2 serological assays using sera of vaccinated subjects ????Clin Chim Acta http://www.kidney.de/pget.php?&tw=1&pmid=34425105 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34425105 #FOAvip English Wikipedia <ref>{{Cite pmid|34425105}}</ref> Harmonization of six quantitative SARS-CoV-2 serological assays using sera of vaccinated subjects #MMPMID34425105 Ferrari D; Clementi N; Spano SM; Albitar-Nehme S; Ranno S; Colombini A; Criscuolo E; Di Resta C; Tomaiuolo R; Vigano M; Mancini N; De Vecchi E; Locatelli M; Mangia A; Perno CF; Banfi G Clin Chim Acta 2021[Nov]; 522 (ä): 144-151 PMID34425105show ga BACKGROUND AND AIMS: Vaccines, to limit SARS-CoV-2 infection, were produced and reliable assays are needed for their evaluation. The WHO produced an International-Standard (WHO-IS) to facilitate the standardization/comparison of serological methods. The WHO-IS, produced in limited amount, was never tested for reproducibility. This study aims at developing a reproducible and accessible working standard (WS) to complement the WHO-IS. MATERIALS AND METHODS: Sera from vaccinated individuals were used to produce the WSs. The WHO-IS, the WSs and single serum samples (n = 48) were tested on 6 quantitative serological devices. Neutralization assays were performed for the 48 samples and compared with their antibody titers. RESULTS: The WS carry an antibody titer 20-fold higher than the WHO-IS. It was reproducible, showed both good linearity and insignificant intra- and inter-laboratory variability. However, the WSs behave differently from the WHO-IS. Analysis of the 48 samples showed that single correlation factors are not sufficient to harmonize results from different assays. Yet, all the devices predict neutralization activity based on the antibody titer. CONCLUSIONS: A reproducible and highly concentrated WS, specific for IgG against SARS-CoV-2 Spike-glycoprotein was produced. Such characteristics make it particularly suited for the harmonization of commercially available assays and the consequent evaluation of post-vaccinated individuals. |*COVID-19[MESH] |*SARS-CoV-2[MESH] |Antibodies, Neutralizing[MESH] |Antibodies, Viral[MESH] |Humans[MESH] |Neutralization Tests[MESH]Harmonization of six quantitative SARS-CoV-2 serological assays using (epmc).pdf DeepDyve Pubget Overpricing