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10.1016/j.biosx.2021.100076 http://scihub22266oqcxt.onion/10.1016/j.biosx.2021.100076 34423284!8364207!34423284     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Biosens+Bioelectron+X 2021 ; 9 (ä): 100076 Nephropedia Template TP {{tp|p=34423284|t=2021. A paper-based colorimetric molecular test for SARS-CoV-2 in saliva |pdf=|usr=}}{{34423284}} gab.com Text A paper-based colorimetric molecular test for SARS-CoV-2 in saliva JO: Biosens Bioelectron X http://www.kidney.de/pget.php?&tw=1&pmid=34423284 #FOAvip Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/muL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex(R) backing via an ARclean(R) double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets. Twit Text FOAVip A paper-based colorimetric molecular test for SARS-CoV-2 in saliva ????Biosens Bioelectron X http://www.kidney.de/pget.php?&tw=1&pmid=34423284 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34423284 #FOAvip English Wikipedia <ref>{{Cite pmid|34423284}}</ref> A paper-based colorimetric molecular test for SARS-CoV-2 in saliva #MMPMID34423284 Davidson JL; Wang J; Maruthamuthu MK; Dextre A; Pascual-Garrigos A; Mohan S; Putikam SVS; Osman FOI; McChesney D; Seville J; Verma MS Biosens Bioelectron X 2021[Dec]; 9 (ä): 100076 PMID34423284show ga Herein, we describe the development of a paper-based device to detect nucleic acids of pathogens of interest in complex samples using loop-mediated isothermal amplification (LAMP) by producing a colorimetric response visible to the human eye. To demonstrate the utility of this device in emerging public health emergencies, we developed and optimized our device to detect SARS-CoV-2 in human saliva without preprocessing. The resulting device was capable of detecting the virus within 60 min and had an analytical sensitivity of 97% and a specificity of 100% with a limit of detection of 200 genomic copies/muL of patient saliva using image analysis. The device consists of a configurable number of reaction zones constructed of Grade 222 chromatography paper separated by 20 mil polystyrene spacers attached to a Melinex(R) backing via an ARclean(R) double-sided adhesive. The resulting device is easily configurable to detect multiple targets and has the potential to detect a variety of pathogens simply by changing the LAMP primer sets. äA paper-based colorimetric molecular test for SARS-CoV-2 in saliva (epmc).pdf DeepDyve Pubget Overpricing