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10.3892/ijmm.2021.5022 http://scihub22266oqcxt.onion/10.3892/ijmm.2021.5022 34414454!8416148!34414454     free     free     free Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 213.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 247.2 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 280.79999999999995 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 314.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 314.4 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Int+J+Mol+Med 2021 ; 48 (4): ä Nephropedia Template TP {{tp|p=34414454|t=2021. MicroRNA?28?3p inhibits angiotensin?converting enzyme 2 ectodomain shedding in 293T cells treated with the spike protein of severe acute respiratory syndrome coronavirus 2 by targeting A disintegrin and metalloproteinase 17 |pdf=|usr=}}{{34414454}} gab.com Text MicroRNA 28 3p inhibits angiotensin converting enzyme 2 ectodomain shedding in 293T cells treated with the spike protein of severe acute respiratory syndrome coronavirus 2 by targeting A disintegrin and metalloproteinase 17 JO: Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=34414454 #FOAvip Severe acute respiratory syndrome coronavirus 2 (SARS?CoV?2) is the virus that causes coronavirus disease 2019. Angiotensin?converting enzyme 2 (ACE2) is the SARS?CoV binding site and is ubiquitously expressed in endothelial cells of several organs, with the highest levels in the cardiovascular system, kidney and lungs. A disintegrin and metalloproteinase 17 (ADAM17) is involved in ectodomain shedding of ACE2. In the present study, reverse?transcription?quantitative PCR, transfection, TUNNEL assay, dual?luciferase activity assay and western blotting were conducted to investigate the effects of microRNA (miR)?28?3p on ADAM17?dependent shedding of the ACE2 ectodomain following treatment with the spike protein (S?protein) of SARS?CoV?2. It was found that miR?28?3p was significantly downregulated in 293T cells treated with 100 ng/ml of S?protein for 24 h at 37 C, which led to upregulation of ADAM17. In addition, the expression of ADAM17 and miR?28?3p were negatively correlated based on Pearson's correlation test in 293T cells treated with S?protein for 24 h. Overexpression of miR?28?3p and inhibition of ADAM17 regulated 293T cell viability, apoptosis and ACE2 ectodomain shedding. It was also demonstrated that ADAM17 was the target gene of miR?28?3p and that miR?28?3p negatively regulated ADAM17 expression. Notably, the inhibition of ADAM17 expression blocked the effects of miR?28?3p inhibitor on proliferation, apoptosis and ACE2 ectodomain shedding in 293T cells treated with S?protein. The findings of the present study suggested that miR?28?3p inhibits ADAM17?dependent ACE2 ectodomain shedding in 293T cells treated with the S?protein of SARS?CoV?2, which suggested the potential therapeutic role of miR?28?3p mimic in the prevention and treatment of patients with SARS?CoV?2. Twit Text FOAVip MicroRNA 28 3p inhibits angiotensin converting enzyme 2 ectodomain shedding in 293T cells treated with the spike protein of severe acute respiratory syndrome coronavirus 2 by targeting A disintegrin and metalloproteinase 17 ????Int J Mol Med http://www.kidney.de/pget.php?&tw=1&pmid=34414454 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34414454 #FOAvip English Wikipedia <ref>{{Cite pmid|34414454}}</ref> MicroRNA?28?3p inhibits angiotensin?converting enzyme 2 ectodomain shedding in 293T cells treated with the spike protein of severe acute respiratory syndrome coronavirus 2 by targeting A disintegrin and metalloproteinase 17 #MMPMID34414454 Xu Y; Li Y Int J Mol Med 2021[Oct]; 48 (4): ä PMID34414454show ga Severe acute respiratory syndrome coronavirus 2 (SARS?CoV?2) is the virus that causes coronavirus disease 2019. Angiotensin?converting enzyme 2 (ACE2) is the SARS?CoV binding site and is ubiquitously expressed in endothelial cells of several organs, with the highest levels in the cardiovascular system, kidney and lungs. A disintegrin and metalloproteinase 17 (ADAM17) is involved in ectodomain shedding of ACE2. In the present study, reverse?transcription?quantitative PCR, transfection, TUNNEL assay, dual?luciferase activity assay and western blotting were conducted to investigate the effects of microRNA (miR)?28?3p on ADAM17?dependent shedding of the ACE2 ectodomain following treatment with the spike protein (S?protein) of SARS?CoV?2. It was found that miR?28?3p was significantly downregulated in 293T cells treated with 100 ng/ml of S?protein for 24 h at 37 C, which led to upregulation of ADAM17. In addition, the expression of ADAM17 and miR?28?3p were negatively correlated based on Pearson's correlation test in 293T cells treated with S?protein for 24 h. Overexpression of miR?28?3p and inhibition of ADAM17 regulated 293T cell viability, apoptosis and ACE2 ectodomain shedding. It was also demonstrated that ADAM17 was the target gene of miR?28?3p and that miR?28?3p negatively regulated ADAM17 expression. Notably, the inhibition of ADAM17 expression blocked the effects of miR?28?3p inhibitor on proliferation, apoptosis and ACE2 ectodomain shedding in 293T cells treated with S?protein. The findings of the present study suggested that miR?28?3p inhibits ADAM17?dependent ACE2 ectodomain shedding in 293T cells treated with the S?protein of SARS?CoV?2, which suggested the potential therapeutic role of miR?28?3p mimic in the prevention and treatment of patients with SARS?CoV?2. |*Gene Expression Profiling[MESH] |ADAM17 Protein/*metabolism[MESH] |Angiotensin-Converting Enzyme 2/*chemistry[MESH] |Apoptosis[MESH] |COVID-19/*immunology/virology[MESH] |Cell Proliferation[MESH] |Cell Survival[MESH] |Gene Expression Regulation, Viral[MESH] |HEK293 Cells[MESH] |Humans[MESH] |MicroRNAs/antagonists & inhibitors/*physiology[MESH]MicroRNA?28?3p inhibits angiotensin?converting enzyme 2 ectodomain she(epmc).pdf DeepDyve Pubget Overpricing