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10.1007/978-1-0716-1518-8_8 http://scihub22266oqcxt.onion/10.1007/978-1-0716-1518-8_8 34410643!ä!34410643 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 211.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Methods+Mol+Biol 2021 ; 2327 (ä): 119-137 Nephropedia Template TP {{tp|p=34410643|t=2021. Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment Approach for SARS-CoV-2 |pdf=|usr=}}{{34410643}} gab.com Text Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment Approach for SARS-CoV-2 JO: Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34410643 #FOAvip Outbreak analysis and transmission surveillance of viruses can be performed via whole-genome sequencing after viral isolation. Such techniques have recently been applied to characterize and monitor SARS-CoV-2 , the etiological agent of the COVID-19 pandemic. However, the isolation and culture of SARS-CoV-2 is time consuming and requires biosafety level 3 containment, which is not ideal for many resource-constrained settings. An alternate method, bait capture allows target enrichment and sequencing of the entire SARS-CoV-2 genome eliminating the need for viral culture. This method uses a set of hybridization probes known as "baits" that span the genome and provide sensitive, accurate, and minimal off-target hybridization. Baits can be designed to detect any known virus or bacteria in a wide variety of specimen types, including oral secretions. The bait capture method presented herein allows the whole genome of SARS-CoV-2 in saliva to be sequenced without the need to culture and provides an outline of bait design and bioinformatic analysis to guide a bioinformatician. Twit Text FOAVip Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment Approach for SARS-CoV-2 ????Methods Mol Biol http://www.kidney.de/pget.php?&tw=1&pmid=34410643 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34410643 #FOAvip English Wikipedia <ref>{{Cite pmid|34410643}}</ref> Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment Approach for SARS-CoV-2 #MMPMID34410643 Speicher DJ; Nasir JA; Zhou P; Anderson DE Methods Mol Biol 2021[]; 2327 (ä): 119-137 PMID34410643show ga Outbreak analysis and transmission surveillance of viruses can be performed via whole-genome sequencing after viral isolation. Such techniques have recently been applied to characterize and monitor SARS-CoV-2 , the etiological agent of the COVID-19 pandemic. However, the isolation and culture of SARS-CoV-2 is time consuming and requires biosafety level 3 containment, which is not ideal for many resource-constrained settings. An alternate method, bait capture allows target enrichment and sequencing of the entire SARS-CoV-2 genome eliminating the need for viral culture. This method uses a set of hybridization probes known as "baits" that span the genome and provide sensitive, accurate, and minimal off-target hybridization. Baits can be designed to detect any known virus or bacteria in a wide variety of specimen types, including oral secretions. The bait capture method presented herein allows the whole genome of SARS-CoV-2 in saliva to be sequenced without the need to culture and provides an outline of bait design and bioinformatic analysis to guide a bioinformatician. |*Genome, Viral[MESH] |Computational Biology[MESH] |DNA, Complementary/genetics[MESH] |Humans[MESH] |Molecular Probes/genetics[MESH] |Polymerase Chain Reaction/methods[MESH] |SARS-CoV-2/*genetics/isolation & purification[MESH] |Saliva/*virology[MESH] |Specimen Handling/methods[MESH] |Streptavidin[MESH]Whole-Genome Sequencing of Pathogens in Saliva : A Target-Enrichment A(epmc).pdf DeepDyve Pubget Overpricing