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10.1093/eurheartj/ehab506 http://scihub22266oqcxt.onion/10.1093/eurheartj/ehab506 34405870!8385969!34405870     free     free     free Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 233.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Eur+Heart+J 2021 ; 42 (39): 4064-4072 Nephropedia Template TP {{tp|p=34405870|t=2021. Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-induced thrombocytopenia |pdf=|usr=}}{{34405870}} gab.com Text Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-induced thrombocytopenia JO: Eur Heart J http://www.kidney.de/pget.php?&tw=1&pmid=34405870 #FOAvip AIMS: We recently reported five cases of vaccine-induced immune thrombotic thrombocytopenia (VITT) 7-10 days after receiving the first dose of the ChAdOx1 nCoV-19 adenoviral vector vaccine against corona virus disease 2019 (COVID-19). We aimed to investigate the pathogenic immunological responses operating in these patients. METHODS AND RESULTS: We assessed circulating inflammatory markers by immune assays and immune cell phenotyping by flow cytometry analyses and performed immunoprecipitation with anti-platelet factor (PF)4 antibody in plasma samples followed by mass spectrometry from all five patients. A thrombus was retrieved from the sinus sagittal superior of one patient and analysed by immunohistochemistry and flow cytometry. Precipitated immune complexes revealed multiple innate immune pathway triggers for platelet and leucocyte activation. Plasma contained increased levels of innate immune response cytokines and markers of systemic inflammation, extensive degranulation of neutrophils, and tissue and endothelial damage. Blood analyses showed activation of neutrophils and increased levels of circulating H3Cit, dsDNA, and myeloperoxidase-DNA complex. The thrombus had extensive infiltration of neutrophils, formation of neutrophil extracellular traps (NETs), and IgG deposits. CONCLUSIONS: The results show that anti-PF4/polyanion IgG-mediated thrombus formation in VITT patients is accompanied by a massive innate immune activation and particularly the fulminant activation of neutrophils including NETosis. These results provide novel data on the immune response in this rare adenoviral vector-induced VITT. Twit Text FOAVip Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-induced thrombocytopenia ????Eur Heart J http://www.kidney.de/pget.php?&tw=1&pmid=34405870 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34405870 #FOAvip English Wikipedia <ref>{{Cite pmid|34405870}}</ref> Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-induced thrombocytopenia #MMPMID34405870 Holm S; Kared H; Michelsen AE; Kong XY; Dahl TB; Schultz NH; Nyman TA; Fladeby C; Seljeflot I; Ueland T; Stensland M; Mjaaland S; Goll GL; Nissen-Meyer LS; Aukrust P; Skagen K; Gregersen I; Skjelland M; Holme PA; Munthe LA; Halvorsen B Eur Heart J 2021[Oct]; 42 (39): 4064-4072 PMID34405870show ga AIMS: We recently reported five cases of vaccine-induced immune thrombotic thrombocytopenia (VITT) 7-10 days after receiving the first dose of the ChAdOx1 nCoV-19 adenoviral vector vaccine against corona virus disease 2019 (COVID-19). We aimed to investigate the pathogenic immunological responses operating in these patients. METHODS AND RESULTS: We assessed circulating inflammatory markers by immune assays and immune cell phenotyping by flow cytometry analyses and performed immunoprecipitation with anti-platelet factor (PF)4 antibody in plasma samples followed by mass spectrometry from all five patients. A thrombus was retrieved from the sinus sagittal superior of one patient and analysed by immunohistochemistry and flow cytometry. Precipitated immune complexes revealed multiple innate immune pathway triggers for platelet and leucocyte activation. Plasma contained increased levels of innate immune response cytokines and markers of systemic inflammation, extensive degranulation of neutrophils, and tissue and endothelial damage. Blood analyses showed activation of neutrophils and increased levels of circulating H3Cit, dsDNA, and myeloperoxidase-DNA complex. The thrombus had extensive infiltration of neutrophils, formation of neutrophil extracellular traps (NETs), and IgG deposits. CONCLUSIONS: The results show that anti-PF4/polyanion IgG-mediated thrombus formation in VITT patients is accompanied by a massive innate immune activation and particularly the fulminant activation of neutrophils including NETosis. These results provide novel data on the immune response in this rare adenoviral vector-induced VITT. |*COVID-19[MESH] |*Thrombocytopenia[MESH] |*Vaccines[MESH] |Antigen-Antibody Complex[MESH] |COVID-19 Vaccines[MESH] |ChAdOx1 nCoV-19[MESH] |Humans[MESH] |Immunity, Innate[MESH]Immune complexes, innate immunity, and NETosis in ChAdOx1 vaccine-indu(epmc).pdf DeepDyve Pubget Overpricing