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10.1016/j.xpro.2021.100781 http://scihub22266oqcxt.onion/10.1016/j.xpro.2021.100781 34405154!8361205!34405154     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       STAR+Protoc 2021 ; 2 (4): 100781 Nephropedia Template TP {{tp|p=34405154|t=2021. Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins |pdf=|usr=}}{{34405154}} gab.com Text Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins JO: STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34405154 #FOAvip We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021). Twit Text FOAVip Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins ????STAR Protoc http://www.kidney.de/pget.php?&tw=1&pmid=34405154 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34405154 #FOAvip English Wikipedia <ref>{{Cite pmid|34405154}}</ref> Luciferase reporter assays to monitor interferon signaling modulation by SARS-CoV-2 proteins #MMPMID34405154 Hirschenberger M; Hayn M; Laliberte A; Koepke L; Kirchhoff F; Sparrer KMJ STAR Protoc 2021[Dec]; 2 (4): 100781 PMID34405154show ga We present a protocol for analyzing the impact of SARS-CoV-2 proteins in interferon signaling using luciferase reporter assays. Here, the induction of defined promoters can be quantitatively assessed with high sensitivity and broad linear range. The results are similar to those obtained using qPCR to measure endogenous mRNA induction. The assay requires stringent normalization and confirmation of the results in more physiological settings. The protocol is adaptable for other viruses and other innate immune stimuli. For complete details on the use and execution of this protocol, please refer to Hayn et al. (2021). |Antiviral Agents/pharmacology[MESH] |COVID-19 Drug Treatment[MESH] |COVID-19/metabolism/*pathology/virology[MESH] |Gene Expression Regulation, Viral/*drug effects[MESH] |Humans[MESH] |Interferons/*pharmacology[MESH] |Luciferases/genetics/*metabolism[MESH] |Promoter Regions, Genetic[MESH] |RNA, Messenger/genetics/*metabolism[MESH] |SARS-CoV-2/drug effects/*metabolism[MESH]Luciferase reporter assays to monitor interferon signaling modulation (epmc).pdf DeepDyve Pubget Overpricing