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10.1016/j.cmi.2021.08.009 http://scihub22266oqcxt.onion/10.1016/j.cmi.2021.08.009 34400343!8360988!34400343     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34400343&cmd=llinks): Failed to open stream: HTTP request failed! 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Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19 |pdf=|usr=}}{{34400343}} gab.com Text Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19 JO: Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34400343 #FOAvip OBJECTIVES: The development of a rapid diagnostic test for viable SARS-CoV-2 is important for infection control. Real-time RT-PCR assays detect non-viable virus, and cell culture differentiates viable virus but it takes several weeks and is labour-intensive. Subgenomic RNAs may reflect replication-competent virus. We therefore evaluated the usefulness of subgenomic RNAs for diagnosing viable SARS-CoV-2 in patients with COVID-19. METHODS: Patients with various severities of confirmed COVID-19 were enrolled at a tertiary hospital between February and December 2020. RT-PCR assay results for genomic and subgenomic RNA of SARS-CoV-2 from nasopharyngeal swab, sputum and saliva specimens were compared with cell culture results. RESULTS: A total 189 specimens from 20 COVID-19 patients were tested in genomic and subgenomic PCR assays and cultured on Vero cells. Of these 189 samples, 62 (33%) gave positive culture results, 93 (49%) negative results and the remaining 34 (18%) indeterminate results. Compared with cell culture results, the sensitivities of genomic RNA and subgenomic RNA of the N and S genes were comparable at 100%, but the specificity of subgenomic RNA (N, 65% and S, 68%) was higher than that of genomic RNA (N, 23% and S, 17%, p < 0.001). The mean durations of positive culture and subgenomic RNA were 11.39 +/- 10.34 and 13.75 +/- 11.22 days after symptom onset (p 0.437), respectively, while that of genomic RNA was 22.85 +/- 11.83 days after symptom onset (p < 0.001). DISCUSSION: Our comparison of subgenomic RNA detection with symptom duration and SARS-CoV-2 culture positivity provides a significant advancement on the transmissibility-based approach beyond the detection of SARS-CoV-2 genomic RNA, and warrants further studies on the development of better diagnostic strategy. Twit Text FOAVip Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19 ????Clin Microbiol Infect http://www.kidney.de/pget.php?&tw=1&pmid=34400343 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34400343 #FOAvip English Wikipedia <ref>{{Cite pmid|34400343}}</ref> Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2 in patients with COVID-19 #MMPMID34400343 Kim JY; Bae JY; Bae S; Cha HH; Kwon JS; Suh MH; Lee HJ; Jung J; Kim MJ; Cui C; Park H; Lee J; Park MS; Kim SH Clin Microbiol Infect 2022[Jan]; 28 (1): 101-106 PMID34400343show ga OBJECTIVES: The development of a rapid diagnostic test for viable SARS-CoV-2 is important for infection control. Real-time RT-PCR assays detect non-viable virus, and cell culture differentiates viable virus but it takes several weeks and is labour-intensive. Subgenomic RNAs may reflect replication-competent virus. We therefore evaluated the usefulness of subgenomic RNAs for diagnosing viable SARS-CoV-2 in patients with COVID-19. METHODS: Patients with various severities of confirmed COVID-19 were enrolled at a tertiary hospital between February and December 2020. RT-PCR assay results for genomic and subgenomic RNA of SARS-CoV-2 from nasopharyngeal swab, sputum and saliva specimens were compared with cell culture results. RESULTS: A total 189 specimens from 20 COVID-19 patients were tested in genomic and subgenomic PCR assays and cultured on Vero cells. Of these 189 samples, 62 (33%) gave positive culture results, 93 (49%) negative results and the remaining 34 (18%) indeterminate results. Compared with cell culture results, the sensitivities of genomic RNA and subgenomic RNA of the N and S genes were comparable at 100%, but the specificity of subgenomic RNA (N, 65% and S, 68%) was higher than that of genomic RNA (N, 23% and S, 17%, p < 0.001). The mean durations of positive culture and subgenomic RNA were 11.39 +/- 10.34 and 13.75 +/- 11.22 days after symptom onset (p 0.437), respectively, while that of genomic RNA was 22.85 +/- 11.83 days after symptom onset (p < 0.001). DISCUSSION: Our comparison of subgenomic RNA detection with symptom duration and SARS-CoV-2 culture positivity provides a significant advancement on the transmissibility-based approach beyond the detection of SARS-CoV-2 genomic RNA, and warrants further studies on the development of better diagnostic strategy. |*COVID-19/diagnosis[MESH] |*SARS-CoV-2/isolation & purification[MESH] |Animals[MESH] |COVID-19 Nucleic Acid Testing/*methods[MESH] |Chlorocebus aethiops[MESH] |Humans[MESH] |Polymerase Chain Reaction[MESH] |RNA, Viral/genetics/*isolation & purification[MESH] |Sensitivity and Specificity[MESH]Diagnostic usefulness of subgenomic RNA detection of viable SARS-CoV-2(epmc).pdf DeepDyve Pubget Overpricing