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10.3389/fimmu.2021.697840 http://scihub22266oqcxt.onion/10.3389/fimmu.2021.697840 34394090!8356644!34394090     free     free     free Warning: file_get_contents(https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pubmed&id=34394090&cmd=llinks): Failed to open stream: HTTP request failed! 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CD169 Defines Activated CD14(+) Monocytes With Enhanced CD8(+) T Cell Activation Capacity |pdf=|usr=}}{{34394090}} gab.com Text CD169 Defines Activated CD14(+) Monocytes With Enhanced CD8(+) T Cell Activation Capacity JO: Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34394090 #FOAvip Monocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role in T cell stimulation is not well defined. In inflammatory conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)-regulated protein. However, little is known about the phenotype and function of these CD169(+) monocytes. Here, we have investigated the phenotype of human CD169(+) monocytes in different diseases, their capacity to activate CD8(+) T cells, and the potential for a targeted-vaccination approach. Using spectral flow cytometry, we detected CD169 expression by CD14(+) CD16(-) classical and CD14(+) CD16(+) intermediate monocytes and unbiased analysis showed that they were distinct from dendritic cells, including the recently described CD14-expressing DC3. CD169(+) monocytes expressed higher levels of co-stimulatory and HLA molecules, suggesting an increased activation state. IFNalpha treatment highly upregulated CD169 expression on CD14(+) monocytes and boosted their capacity to cross-present antigen to CD8(+) T cells. Furthermore, we observed CD169(+) monocytes in virally-infected patients, including in the blood and bronchoalveolar lavage fluid of COVID-19 patients, as well as in the blood of patients with different types of cancers. Finally, we evaluated two CD169-targeting nanovaccine platforms, antibody-based and liposome-based, and we showed that CD169(+) monocytes efficiently presented tumor-associated peptides gp100 and WT1 to antigen-specific CD8(+) T cells. In conclusion, our data indicate that CD169(+) monocytes are activated monocytes with enhanced CD8(+) T cell stimulatory capacity and that they emerge as an interesting target in nanovaccine strategies, because of their presence in health and different diseases. Twit Text FOAVip CD169 Defines Activated CD14(+) Monocytes With Enhanced CD8(+) T Cell Activation Capacity ????Front Immunol http://www.kidney.de/pget.php?&tw=1&pmid=34394090 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34394090 #FOAvip English Wikipedia <ref>{{Cite pmid|34394090}}</ref> CD169 Defines Activated CD14(+) Monocytes With Enhanced CD8(+) T Cell Activation Capacity #MMPMID34394090 Affandi AJ; Olesek K; Grabowska J; Nijen Twilhaar MK; Rodriguez E; Saris A; Zwart ES; Nossent EJ; Kalay H; de Kok M; Kazemier G; Stockl J; van den Eertwegh AJM; de Gruijl TD; Garcia-Vallejo JJ; Storm G; van Kooyk Y; den Haan JMM Front Immunol 2021[]; 12 (ä): 697840 PMID34394090show ga Monocytes are antigen-presenting cells (APCs) that play diverse roles in promoting or regulating inflammatory responses, but their role in T cell stimulation is not well defined. In inflammatory conditions, monocytes frequently show increased expression of CD169/Siglec-1, a type-I interferon (IFN-I)-regulated protein. However, little is known about the phenotype and function of these CD169(+) monocytes. Here, we have investigated the phenotype of human CD169(+) monocytes in different diseases, their capacity to activate CD8(+) T cells, and the potential for a targeted-vaccination approach. Using spectral flow cytometry, we detected CD169 expression by CD14(+) CD16(-) classical and CD14(+) CD16(+) intermediate monocytes and unbiased analysis showed that they were distinct from dendritic cells, including the recently described CD14-expressing DC3. CD169(+) monocytes expressed higher levels of co-stimulatory and HLA molecules, suggesting an increased activation state. IFNalpha treatment highly upregulated CD169 expression on CD14(+) monocytes and boosted their capacity to cross-present antigen to CD8(+) T cells. Furthermore, we observed CD169(+) monocytes in virally-infected patients, including in the blood and bronchoalveolar lavage fluid of COVID-19 patients, as well as in the blood of patients with different types of cancers. Finally, we evaluated two CD169-targeting nanovaccine platforms, antibody-based and liposome-based, and we showed that CD169(+) monocytes efficiently presented tumor-associated peptides gp100 and WT1 to antigen-specific CD8(+) T cells. In conclusion, our data indicate that CD169(+) monocytes are activated monocytes with enhanced CD8(+) T cell stimulatory capacity and that they emerge as an interesting target in nanovaccine strategies, because of their presence in health and different diseases. |Antigen Presentation/*immunology[MESH] |CD8-Positive T-Lymphocytes/*immunology[MESH] |COVID-19/immunology[MESH] |Carcinoma, Pancreatic Ductal/immunology[MESH] |Cells, Cultured[MESH] |Flow Cytometry[MESH] |Humans[MESH] |Influenza, Human/immunology[MESH] |Interferon-alpha/pharmacology[MESH] |Lipopolysaccharide Receptors/metabolism[MESH] |Lung Neoplasms/immunology[MESH] |Lymphocyte Activation/*immunology[MESH] |Monocytes/*immunology[MESH] |Pancreatic Neoplasms/immunology[MESH] |SARS-CoV-2/immunology[MESH]CD169 Defines Activated CD14(+) Monocytes With Enhanced CD8(+) T Cell (epmc).pdf DeepDyve Pubget Overpricing