Use my Search Websuite to scan PubMed, PMCentral, Journal Hosts and Journal Archives, FullText. Kick-your-searchterm to multiple Engines kick-your-query now !>

A dictionary by aggregated review articles of nephrology, medicine and the life sciences Your one-stop-run pathway from word to the immediate pdf of peer-reviewed on-topic knowledge.

10.1016/j.diagmicrobio.2021.115508 http://scihub22266oqcxt.onion/10.1016/j.diagmicrobio.2021.115508 34391075!8299291!34391075     free     free     free Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534 Deprecated: Implicit conversion from float 229.6 to int loses precision in C:\Inetpub\vhosts\kidney.de\httpdocs\pget.php on line 534       Diagn+Microbiol+Infect+Dis 2021 ; 101 (3): 115508 Nephropedia Template TP {{tp|p=34391075|t=2021. Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus |pdf=|usr=}}{{34391075}} gab.com Text Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus JO: Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34391075 #FOAvip We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus, to sequence the SARS-CoV-2 genome and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA between May and July, 2020), in which SARS-CoV-2 had been detected by a PCR assay. For 48 of the samples, we also applied the ARTIC protocol. Of the 70 ONETest libraries, 45 (64%) had a (near-)complete sequence (>29,000 bases and >90% covered by >9 reads). Of the 48 ARTIC libraries, 25 (52%) had a (near-)complete sequence. In 19 out of 25 (76%) samples in which both the ONETest and ARTIC yielded (near-)complete sequences, the lineages assigned were identical. As a target capture approach, the ONETest is less prone to loss of sequence coverage than amplicon approaches, and thus can provide complete genomic information more often to track and monitor SARS-CoV-2 variants. Twit Text FOAVip Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus ????Diagn Microbiol Infect Dis http://www.kidney.de/pget.php?&tw=1&pmid=34391075 #FOAvip Twit Text # Free Paper on # # # # # LINK http://www.kidney.de/pget.php?&tw=1&pmid=34391075 #FOAvip English Wikipedia <ref>{{Cite pmid|34391075}}</ref> Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coronaviruses Plus #MMPMID34391075 Zhan SH; Alamouti SM; Daneshpajouh H; Kwok BS; Lee MH; Khattra J; Houck HJ; Rand KH Diagn Microbiol Infect Dis 2021[Nov]; 101 (3): 115508 PMID34391075show ga We introduce a target capture next-generation sequencing methodology, the ONETest Coronaviruses Plus, to sequence the SARS-CoV-2 genome and select loci of other respiratory viruses. We applied the ONETest on 70 respiratory samples (collected in Florida, USA between May and July, 2020), in which SARS-CoV-2 had been detected by a PCR assay. For 48 of the samples, we also applied the ARTIC protocol. Of the 70 ONETest libraries, 45 (64%) had a (near-)complete sequence (>29,000 bases and >90% covered by >9 reads). Of the 48 ARTIC libraries, 25 (52%) had a (near-)complete sequence. In 19 out of 25 (76%) samples in which both the ONETest and ARTIC yielded (near-)complete sequences, the lineages assigned were identical. As a target capture approach, the ONETest is less prone to loss of sequence coverage than amplicon approaches, and thus can provide complete genomic information more often to track and monitor SARS-CoV-2 variants. |*Genome, Viral[MESH] |Adult[MESH] |COVID-19/*diagnosis/*virology[MESH] |Female[MESH] |Genomics/*methods[MESH] |Humans[MESH] |Male[MESH] |Middle Aged[MESH] |Polymerase Chain Reaction/methods[MESH] |Retrospective Studies[MESH]Target capture sequencing of SARS-CoV-2 genomes using the ONETest Coro(epmc).pdf DeepDyve Pubget Overpricing